Detailed information of TA system TA201837

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1295296..1296213
• Replicon: chromosome
• Accession: NZ_CP074348
• Organism: Bacillus velezensis strain SW5

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: KHA74_RS06600
• Protein ID: WP_094032372.1
• Coordinates: 1295467..1296213 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: KHA74_RS06595
• Protein ID: WP_003154807.1
• Coordinates: 1295296..1295466 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHA74_RS06555 (1290509)	1290509..1292098	+	1590	WP_053573489.1	hypothetical protein	-
KHA74_RS06560 (1292111)	1292111..1292536	+	426	WP_079892045.1	hypothetical protein	-
KHA74_RS06565 (1292541)	1292541..1292738	+	198	WP_012117366.1	XkdX family protein	-
KHA74_RS06570 (1292795)	1292795..1293556	+	762	WP_094032370.1	phage portal protein	-
KHA74_RS06575 (1293608)	1293608..1293871	+	264	WP_003154815.1	hemolysin XhlA family protein	-
KHA74_RS06580 (1293885)	1293885..1294148	+	264	WP_094032371.1	phage holin	-
KHA74_RS06585 (1294162)	1294162..1295040	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
KHA74_RS06595 (1295296)	1295296..1295466	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
KHA74_RS06600 (1295467)	1295467..1296213	-	747	WP_094032372.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
KHA74_RS06605 (1296318)	1296318..1297316	-	999	WP_020955696.1	inorganic phosphate transporter	-
KHA74_RS06610 (1297329)	1297329..1297946	-	618	WP_003154804.1	DUF47 domain-containing protein	-
KHA74_RS06615 (1298232)	1298232..1299548	-	1317	WP_094032373.1	amino acid permease	-
KHA74_RS06620 (1299871)	1299871..1300821	+	951	WP_094032374.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29061.57 Da
• Isoelectric Point: 4.7755

>T201837 WP_094032372.1 NZ_CP074348:c1296213-1295467 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQADKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T201837 NZ_CP098778:c2689682-2689579 [Enterobacter hormaechei]
GGCAAGGCGATTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT201837 WP_003154807.1 NZ_CP074348:c1295466-1295296 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT201837 NZ_CP098778:2689575-2689775 [Enterobacter hormaechei]
AGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTG
GCATTAATGCAGGCTCAATCGCCTTGCCCTTTAAGAATAGATGACGACGTCAGGTTTTCCAGTCCACAGCAAAAGTGGTC
TGAAAAAAAGCGTCAGAACATCACTAAATGTGAAAAACCGC

Structures

Antitoxin

• AlphaFold DB: I2HQ14