Detailed information of TA system TA201805

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3179892..3181626
• Replicon: chromosome
• Accession: NZ_CP074200
• Organism: Halomonas sp. FeN2

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: KF947_RS14730
• Protein ID: WP_133730890.1
• Coordinates: 3179892..3180536 (+)
• Length: 215 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: KF947_RS14735
• Protein ID: WP_133730889.1
• Coordinates: 3180547..3181626 (+)
• Length: 360 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KF947_RS14710 (KF947_14665)	3175432..3176430	+	999	WP_088698369.1	TRAP transporter substrate-binding protein DctP	-
KF947_RS14715 (KF947_14670)	3176493..3176999	+	507	WP_035576621.1	TRAP transporter small permease	-
KF947_RS14720 (KF947_14675)	3176999..3178312	+	1314	WP_088698368.1	TRAP transporter large permease	-
KF947_RS14725 (KF947_14680)	3178398..3179624	-	1227	WP_227404292.1	zinc metallochaperone GTPase ZigA	-
KF947_RS14730 (KF947_14685)	3179892..3180536	+	645	WP_133730890.1	DUF4433 domain-containing protein	Toxin
KF947_RS14735 (KF947_14690)	3180547..3181626	+	1080	WP_133730889.1	macro domain-containing protein	Antitoxin
KF947_RS14740 (KF947_14695)	3181681..3181956	-	276	WP_133730888.1	TIGR03643 family protein	-
KF947_RS14745 (KF947_14700)	3181975..3183348	-	1374	WP_227404293.1	PepSY-associated TM helix domain-containing protein	-
KF947_RS14750 (KF947_14705)	3183453..3184076	-	624	WP_227404294.1	methyltransferase domain-containing protein	-
KF947_RS14755 (KF947_14710)	3184347..3184694	-	348	WP_227404295.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (14-214)

Antitoxin

• Macro domain (18-127)

Sequences

Toxin

Protein

• Length: 215 a.a.
• Molecular weight: 24400.90 Da
• Isoelectric Point: 7.5414

>T201805 WP_133730890.1 NZ_CP074200:3179892-3180536 [Halomonas sp. FeN2]
MVYKNLNPGKALIWRITHRQNLPWILTNGLHAGHGSRRAPEWVVIGNEELISRRAHRHVPLAPGGVLNDYVPFYFTPFSP
MLYNIHTGRGGVKTVANEDIVILVSSLYKAAELSLPFVFTDRHAYPVTANYFNGLDNLSAIDWPLLQQRNFQRDPDDPEK
VERYQAEALIHKYVPIQALLGAICYTPQLQLELQQQSAELGSALDIHCRPSWYF

Nucleotide

• Length: 645 bp

>T201805 NZ_CP098770:355609-355956 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 360 a.a.
• Molecular weight: 40126.09 Da
• Isoelectric Point: 5.5101

>AT201805 WP_133730889.1 NZ_CP074200:3180547-3181626 [Halomonas sp. FeN2]
MITYTTGNLLDAEVEALVNTVNTVGVMGKGIALMFKERFPANMAAYAQACKAGKVQTGHMFVTETDELMGPRWIVNFPTK
QHWRAKSQLQWIIDGLADLRGFIERNQVRSIAIPPLGAGNGGLDWAEIKPHIEQALGDLQGVEIIVFEPTAKYQNVAKRQ
GVEQLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERAIEAEGIDNPLDLRFEANSYGPYADRLRHLLNGLDGSYLK
SEKRINDCDPLDTIWFNDDKREKVALYLKTEAKAYLPALEKTAELIDGFESPFGMELLATVDWLLSQSQAAPDPASLMEG
VSRWPGGKHSAQRKLKLFDRPNVELALSQLNSIQLKSAS

Nucleotide

• Length: 1080 bp

>AT201805 NZ_CP098770:355956-356255 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA