Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 42433..43169 Replicon plasmid pJBIWA001_4
Accession NZ_CP074186
Organism Raoultella planticola strain JBIWA001

Toxin (Protein)


Gene name tacT Uniprot ID J5UJ49
Locus tag KGP19_RS29185 Protein ID WP_009654334.1
Coordinates 42687..43169 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KGP19_RS29180 Protein ID WP_003026799.1
Coordinates 42433..42699 (+) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGP19_RS29155 (KGP19_29035) 37778..37927 - 150 Protein_47 IS110 family transposase -
KGP19_RS29160 (KGP19_29040) 38013..39038 + 1026 WP_001101446.1 IS110 family transposase -
KGP19_RS29165 (KGP19_29045) 39357..40325 - 969 WP_077266288.1 IS5 family transposase -
KGP19_RS29170 (KGP19_29050) 40620..41645 - 1026 WP_001101446.1 IS110 family transposase -
KGP19_RS29175 (KGP19_29055) 41760..42194 + 435 WP_042928448.1 cell envelope integrity protein TolA -
KGP19_RS29180 (KGP19_29060) 42433..42699 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KGP19_RS29185 (KGP19_29065) 42687..43169 + 483 WP_009654334.1 GNAT family N-acetyltransferase Toxin
KGP19_RS29190 (KGP19_29070) 43369..44013 + 645 Protein_54 ISNCY-like element ISKpn21 family transposase -
KGP19_RS29195 (KGP19_29075) 44077..45227 + 1151 WP_112150150.1 IS3 family transposase -
KGP19_RS29200 (KGP19_29080) 45258..46019 + 762 Protein_56 ISNCY-like element ISKpn21 family transposase -
KGP19_RS29205 (KGP19_29085) 46048..46680 - 633 WP_001567369.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / qnrB91 / mph(A) - 1..102491 102491
- inside IScluster/Tn - - 38013..52779 14766


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(46-149)

Antitoxin

(6-82)


Sequences


Toxin        


Download         Length: 161 a.a.        Molecular weight: 17339.06 Da        Isoelectric Point: 8.7400

>T201798 WP_009654334.1 NZ_CP074186:42687-43169 [Raoultella planticola]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDISLHVKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYVHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T201798 NZ_CP098769:c2755254-2753932 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCTGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT201798 WP_003026799.1 NZ_CP074186:42433-42699 [Raoultella planticola]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT201798 NZ_CP098769:c2755520-2755254 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A2J4QCA1


Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References