Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5872..6397 Replicon plasmid pJBIWA001_3
Accession NZ_CP074185
Organism Raoultella planticola strain JBIWA001

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag KGP19_RS28305 Protein ID WP_013023785.1
Coordinates 5872..6177 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag KGP19_RS28310 Protein ID WP_001568025.1
Coordinates 6179..6397 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGP19_RS28275 (KGP19_28170) 1567..2193 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
KGP19_RS28280 2190..2492 + 303 WP_004197636.1 hypothetical protein -
KGP19_RS28285 (KGP19_28180) 2948..3742 - 795 WP_004197635.1 site-specific integrase -
KGP19_RS28290 (KGP19_28185) 3940..4956 - 1017 WP_017899884.1 hypothetical protein -
KGP19_RS28295 (KGP19_28190) 4967..5281 - 315 WP_053389906.1 hypothetical protein -
KGP19_RS28300 (KGP19_28195) 5308..5703 - 396 WP_017899885.1 hypothetical protein -
KGP19_RS28305 (KGP19_28200) 5872..6177 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
KGP19_RS28310 (KGP19_28205) 6179..6397 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KGP19_RS28315 (KGP19_28210) 7215..7505 - 291 WP_013023783.1 hypothetical protein -
KGP19_RS28320 (KGP19_28215) 7502..8629 - 1128 WP_013023782.1 DUF4238 domain-containing protein -
KGP19_RS28325 (KGP19_28220) 8663..10186 - 1524 WP_017899887.1 hypothetical protein -
KGP19_RS28330 (KGP19_28225) 10462..11241 - 780 WP_013023780.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / blaCTX-M-3 / qnrS1 - 1..110557 110557


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T201797 WP_013023785.1 NZ_CP074185:c6177-5872 [Raoultella planticola]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T201797 NZ_CP098769:2026623-2026725 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT201797 WP_001568025.1 NZ_CP074185:c6397-6179 [Raoultella planticola]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT201797 NZ_CP098769:c2026732-2026587 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References