Detailed information of TA system TA201796

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 5195337..5196115
• Replicon: chromosome
• Accession: NZ_CP074182
• Organism: Raoultella planticola strain JBIWA001

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: A0A443VCT1
• Locus tag: KGP19_RS24670
• Protein ID: WP_057108449.1
• Coordinates: 5195337..5195825 (-)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A1Y0L5F0
• Locus tag: KGP19_RS24675
• Protein ID: WP_038917748.1
• Coordinates: 5195822..5196115 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP19_RS24650 (KGP19_24580)	5191394..5192803	-	1410	WP_057108451.1	N-6 DNA methylase	-
KGP19_RS24655	5192953..5193121	+	169	Protein_4837	IS3 family transposase	-
KGP19_RS24660 (KGP19_24585)	5193376..5193865	+	490	Protein_4838	MobC family replication-relaxation protein	-
KGP19_RS24665 (KGP19_24590)	5194141..5195244	-	1104	WP_064388374.1	hypothetical protein	-
KGP19_RS24670 (KGP19_24595)	5195337..5195825	-	489	WP_057108449.1	GNAT family N-acetyltransferase	Toxin
KGP19_RS24675 (KGP19_24600)	5195822..5196115	-	294	WP_038917748.1	DUF1778 domain-containing protein	Antitoxin
KGP19_RS24680	5196568..5196690	+	123	Protein_4842	DUF4102 domain-containing protein	-
KGP19_RS24685 (KGP19_24605)	5196934..5197785	+	852	WP_064793415.1	SDR family NAD(P)-dependent oxidoreductase	-
KGP19_RS24690 (KGP19_24610)	5197887..5198456	+	570	WP_046853227.1	TetR/AcrR family transcriptional regulator	-
KGP19_RS24695 (KGP19_24615)	5198562..5199482	+	921	WP_032689869.1	SDR family NAD(P)-dependent oxidoreductase	-
KGP19_RS24700 (KGP19_24620)	5199541..5200218	+	678	WP_032698348.1	NAD-dependent epimerase/dehydratase family protein	-
KGP19_RS24705	5200324..5200517	+	194	Protein_4847	DUF4942 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5153135..5196369	43234

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (71-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17572.45 Da
• Isoelectric Point: 7.2150

>T201796 WP_057108449.1 NZ_CP074182:c5195825-5195337 [Raoultella planticola]
MISAPEPLRAEHVLSSFCCGVESMDNWLKLRAMKNQVTGASRTFVSCDDSKVLAYYSLASSAVAMNSAPGRFRRNMPDPI
PVVMLGRLAVDTSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEAREFYLRVGFEPSPIDPMMLMVTLGDLVG
SF

Nucleotide

• Length: 489 bp

>T201796 NZ_CP098769:877064-877471 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10872.44 Da
• Isoelectric Point: 10.1968

>AT201796 WP_038917748.1 NZ_CP074182:c5196115-5195822 [Raoultella planticola]
MPATNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPSPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT201796 NZ_CP098769:876817-877083 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A443VCT1

Antitoxin

• AlphaFold DB: A0A1Y0L5F0