Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4338536..4339155 Replicon chromosome
Accession NZ_CP074182
Organism Raoultella planticola strain JBIWA001

Toxin (Protein)


Gene name Hha Uniprot ID A0A514EV16
Locus tag KGP19_RS20625 Protein ID WP_004858783.1
Coordinates 4338937..4339155 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A514EV88
Locus tag KGP19_RS20620 Protein ID WP_004858785.1
Coordinates 4338536..4338910 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGP19_RS20610 (KGP19_20560) 4333647..4334840 + 1194 WP_032689203.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KGP19_RS20615 (KGP19_20565) 4334863..4338009 + 3147 WP_032689204.1 multidrug efflux RND transporter permease subunit AcrB -
KGP19_RS20620 (KGP19_20570) 4338536..4338910 + 375 WP_004858785.1 Hha toxicity modulator TomB Antitoxin
KGP19_RS20625 (KGP19_20575) 4338937..4339155 + 219 WP_004858783.1 HHA domain-containing protein Toxin
KGP19_RS20630 (KGP19_20580) 4339307..4339873 + 567 WP_032689205.1 maltose O-acetyltransferase -
KGP19_RS20635 4339845..4339979 - 135 WP_223306663.1 hypothetical protein -
KGP19_RS20640 (KGP19_20585) 4339973..4340443 + 471 WP_004858780.1 YlaC family protein -
KGP19_RS20645 (KGP19_20590) 4340418..4341869 - 1452 WP_064793191.1 PLP-dependent aminotransferase family protein -
KGP19_RS20650 (KGP19_20595) 4341971..4342681 + 711 WP_064793190.1 GNAT family protein -
KGP19_RS20655 (KGP19_20600) 4342678..4342818 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
KGP19_RS20660 (KGP19_20605) 4342821..4343081 - 261 WP_032689208.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8570.90 Da        Isoelectric Point: 7.9907

>T201794 WP_004858783.1 NZ_CP074182:4338937-4339155 [Raoultella planticola]
MSGEPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T201794 NZ_CP098769:354402-354749 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14411.19 Da        Isoelectric Point: 4.8989

>AT201794 WP_004858785.1 NZ_CP074182:4338536-4338910 [Raoultella planticola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYAEDNKLIAQLDEYL
DDTFMLFSSYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT201794 NZ_CP098769:354749-355048 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EV16

Antitoxin

Source ID Structure
AlphaFold DB A0A514EV88

References