Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 840154..840811 Replicon chromosome
Accession NZ_CP074182
Organism Raoultella planticola strain JBIWA001

Toxin (Protein)


Gene name cptA Uniprot ID A0A2X2EG10
Locus tag KGP19_RS04080 Protein ID WP_032690815.1
Coordinates 840401..840811 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A514ELX1
Locus tag KGP19_RS04075 Protein ID WP_004867358.1
Coordinates 840154..840420 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGP19_RS04050 (KGP19_04040) 835301..836734 - 1434 WP_032690812.1 6-phospho-beta-glucosidase -
KGP19_RS04055 (KGP19_04045) 836854..837582 - 729 WP_032690813.1 MurR/RpiR family transcriptional regulator -
KGP19_RS04060 (KGP19_04050) 837632..837943 + 312 WP_004867365.1 N(4)-acetylcytidine aminohydrolase -
KGP19_RS04065 (KGP19_04055) 838105..838764 + 660 WP_004867362.1 hemolysin III family protein -
KGP19_RS04070 (KGP19_04060) 838874..839857 - 984 WP_032690814.1 tRNA-modifying protein YgfZ -
KGP19_RS04075 (KGP19_04065) 840154..840420 + 267 WP_004867358.1 FAD assembly factor SdhE Antitoxin
KGP19_RS04080 (KGP19_04070) 840401..840811 + 411 WP_032690815.1 protein YgfX Toxin
KGP19_RS04085 (KGP19_04075) 840817..841338 - 522 WP_032690816.1 flavodoxin FldB -
KGP19_RS04090 (KGP19_04080) 841517..842413 + 897 WP_032690817.1 site-specific tyrosine recombinase XerD -
KGP19_RS04095 (KGP19_04085) 842436..843149 + 714 WP_032690818.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KGP19_RS04100 (KGP19_04090) 843155..844888 + 1734 WP_032696678.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16198.10 Da        Isoelectric Point: 11.3349

>T201784 WP_032690815.1 NZ_CP074182:840401-840811 [Raoultella planticola]
VVLWQSDLRISWRSQWFSLLLHGIVAAIVLLMPWPLSYTPLWLLLLSLVVFDSVRSQRRIHACRGEIKLMTDSRLRWQKA
EWEIVGTPWVINSGMLLRLQDMQTRRRQHLWVAADSMDAKEWRDLRRLVLQKPAQD

Download         Length: 411 bp

>T201784 NZ_CP098758:c3104603-3104385 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10553.05 Da        Isoelectric Point: 4.8432

>AT201784 WP_004867358.1 NZ_CP074182:840154-840420 [Raoultella planticola]
MDITNKARIHWACRRGMRELDISIMPFFEYEYDTLSDEDKQLFIRLLENDDPDLFNWLMNHGEPADAQLQRMVKLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT201784 NZ_CP098758:c3105004-3104630 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2X2EG10

Antitoxin

Source ID Structure
AlphaFold DB A0A514ELX1

References