Detailed information of TA system TA201783

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RHH
• Location: 231..787
• Replicon: plasmid pJBIWA002_4
• Accession: NZ_CP074180
• Organism: Enterobacter asburiae strain JBIWA002

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KGP20_RS25380
• Protein ID: WP_087770201.1
• Coordinates: 497..787 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KGP20_RS25375
• Protein ID: WP_087770202.1
• Coordinates: 231..509 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP20_RS25375 (KGP20_25250)	231..509	+	279	WP_087770202.1	CopG family ribbon-helix-helix protein	Antitoxin
KGP20_RS25380 (KGP20_25255)	497..787	+	291	WP_087770201.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KGP20_RS25385 (KGP20_25260)	1058..1486	+	429	WP_087770200.1	hypothetical protein	-
KGP20_RS25390 (KGP20_25265)	1511..1693	+	183	WP_087770199.1	Rop family plasmid primer RNA-binding protein	-
KGP20_RS25395 (KGP20_25270)	1723..2067	-	345	WP_087770198.1	hypothetical protein	-
KGP20_RS25400 (KGP20_25275)	2073..2807	-	735	WP_223540843.1	MobC family replication-relaxation protein	-
KGP20_RS25405	2807..2980	-	174	WP_223605404.1	hypothetical protein	-
KGP20_RS25410 (KGP20_25280)	2928..4772	-	1845	WP_223605405.1	type IV secretory system conjugative DNA transfer family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..8798	8798

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-93)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11006.37 Da
• Isoelectric Point: 5.8232

>T201783 WP_087770201.1 NZ_CP074180:497-787 [Enterobacter asburiae]
MPSLSWTPQALADVQRLYRFLAPRDLNAARNAITEIRNSVKILAYQPESGRPIEESPSYREWPISFGNSGYVALYRIEDD
AVTILAVRHQREADWS

Nucleotide

• Length: 291 bp

>T201783 NZ_CP098758:2480831-2481364 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10817.97 Da
• Isoelectric Point: 4.9565

>AT201783 WP_087770202.1 NZ_CP074180:231-509 [Enterobacter asburiae]
MATATSIKLDDDLKNRLQHLAESRRRSAHWIMREAITQYVEREEKREAYLRDAQNAWEEFQRTGLHITAEEADEWLAQLA
AGNDVEPPECHR

Nucleotide

• Length: 279 bp

>AT201783 NZ_CP098758:2480554-2480820 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA