Detailed information of TA system TA020177

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB-PIN
• Location: 2105685..2106313
• Replicon: chromosome
• Accession: NC_000917
• Organism: Archaeoglobus fulgidus DSM 4304

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: O30310
• Locus tag: AF_RS11895
• Protein ID: WP_010879846.1
• Coordinates: 2105909..2106313 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O30311
• Locus tag: AF_RS11890
• Protein ID: WP_010879845.1
• Coordinates: 2105685..2105912 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AF_RS13165	2100755..2101048	+	294	WP_048064569.1	rhomboid family intramembrane serine protease	-
AF_RS11855 (AF2353)	2101053..2101358	+	306	WP_010879839.1	hypothetical protein	-
AF_RS11860 (AF2354)	2101462..2101749	+	288	WP_010879840.1	hypothetical protein	-
AF_RS11865 (AF2355)	2102052..2102273	+	222	WP_010879841.1	hypothetical protein	-
AF_RS11870 (AF2356)	2102386..2102829	+	444	WP_010879842.1	EVE domain-containing protein	-
AF_RS11875 (AF2357)	2102819..2103841	+	1023	WP_010879843.1	transglutaminase domain-containing protein	-
AF_RS11880 (AF2358)	2103892..2104962	-	1071	WP_081423287.1	tyrosine-type recombinase/integrase	-
AF_RS12955	2105328..2105540	-	213	Protein_2417	transposase	-
AF_RS11890 (AF2359)	2105685..2105912	+	228	WP_010879845.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AF_RS11895 (AF2360)	2105909..2106313	+	405	WP_010879846.1	PIN domain-containing protein	Toxin
AF_RS11900 (AF2361)	2106330..2107205	-	876	WP_010879847.1	MBL fold metallo-hydrolase	-
AF_RS11905 (AF2362)	2107258..2107743	+	486	WP_010879848.1	hypothetical protein	-
AF_RS11910 (AF2363)	2107785..2109107	-	1323	WP_010879849.1	AIR synthase-related protein	-
AF_RS11915 (AF2364)	2109210..2109974	+	765	WP_010879850.1	ABC transporter ATP-binding protein	-
AF_RS11920 (AF2365)	2109944..2111041	+	1098	WP_048064571.1	SufD family Fe-S cluster assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• SpoVT-AbrB domain (3-49)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15519.06 Da
• Isoelectric Point: 6.7318

>T20177 WP_010879846.1 NC_000917:2105909-2106313 [Archaeoglobus fulgidus DSM 4304]
MKAVIDTNVIVYDTFEDSVFHQEAMQLLDRIDVWVIPTIVIHEYVWVLKSLKVDVKEIKYKVEEYLNHYKTKMVSENKQI
VLSALERIVGGGLSLSRYNDELILAVAGREKISLATFDERLRRQARARGVEVIP

Nucleotide

• Length: 405 bp

>T20177 NC_000917:2105909-2106313 [Archaeoglobus fulgidus DSM 4304]
ATGAAAGCCGTCATAGATACAAATGTTATAGTCTACGACACCTTTGAAGACAGCGTTTTTCATCAGGAAGCCATGCAACT
CCTCGATAGAATTGATGTGTGGGTAATCCCTACGATAGTTATTCACGAGTATGTTTGGGTCCTCAAATCACTGAAGGTGG
ACGTGAAAGAAATCAAATACAAAGTGGAGGAGTATCTTAACCACTATAAAACGAAAATGGTTTCAGAAAATAAGCAAATC
GTTTTGTCAGCACTGGAAAGAATTGTAGGTGGGGGATTAAGCCTTTCAAGGTACAACGACGAGTTGATACTTGCTGTTGC
TGGTAGGGAAAAAATAAGCCTTGCAACCTTCGACGAGAGGTTGAGAAGGCAGGCGAGAGCAAGAGGTGTAGAAGTAATTC
CATAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8464.94 Da
• Isoelectric Point: 10.0936

>AT20177 WP_010879845.1 NC_000917:2105685-2105912 [Archaeoglobus fulgidus DSM 4304]
MKVKVTRNFQITIPAEVRRKMGLKLGDVLEVEYNEEKGEAIIRKLGGERRKLKAGRKLTPDEIEALIAEGMGDNL

Nucleotide

• Length: 228 bp

>AT20177 NC_000917:2105685-2105912 [Archaeoglobus fulgidus DSM 4304]
ATGAAAGTAAAAGTTACTAGGAATTTTCAGATTACTATACCTGCCGAAGTTAGGAGAAAGATGGGATTAAAGCTGGGCGA
TGTGCTTGAAGTTGAATACAACGAGGAGAAGGGAGAGGCCATTATAAGGAAGCTCGGTGGAGAGAGAAGGAAGCTGAAAG
CGGGAAGAAAGCTTACGCCGGACGAGATAGAGGCGCTGATAGCTGAGGGAATGGGCGATAACTTATGA

Structures

Toxin

• AlphaFold DB: A0A075WJC8

Antitoxin

• AlphaFold DB: A0A075WP36