Detailed information of TA system TA201768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 2942096..2942893
• Replicon: chromosome
• Accession: NZ_CP074177
• Organism: Enterobacter asburiae strain JBIWA002

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: KGP20_RS14500
• Protein ID: WP_196350389.1
• Coordinates: 2942393..2942893 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W7PG43
• Locus tag: KGP20_RS14495
• Protein ID: WP_008499829.1
• Coordinates: 2942096..2942365 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP20_RS14465 (KGP20_14410)	2937722..2938165	-	444	WP_032658095.1	helix-turn-helix domain-containing protein	-
KGP20_RS14470 (KGP20_14415)	2938318..2939559	+	1242	WP_223603885.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
KGP20_RS14475 (KGP20_14420)	2939593..2939820	-	228	WP_008499832.1	YccJ family protein	-
KGP20_RS14480 (KGP20_14425)	2939841..2940437	-	597	WP_023311076.1	NAD(P)H:quinone oxidoreductase	-
KGP20_RS14485 (KGP20_14430)	2940829..2940999	+	171	WP_023311077.1	general stress protein	-
KGP20_RS14490 (KGP20_14435)	2941117..2942034	+	918	WP_047742252.1	DMT family transporter	-
KGP20_RS14495 (KGP20_14440)	2942096..2942365	+	270	WP_008499829.1	DUF1778 domain-containing protein	Antitoxin
KGP20_RS14500 (KGP20_14445)	2942393..2942893	+	501	WP_196350389.1	GNAT family N-acetyltransferase	Toxin
KGP20_RS14505 (KGP20_14450)	2942967..2944289	-	1323	WP_223603887.1	pyrimidine utilization transport protein G	-
KGP20_RS14510 (KGP20_14455)	2944311..2944805	-	495	WP_023311082.1	pyrimidine utilization flavin reductase protein F	-
KGP20_RS14515 (KGP20_14460)	2944815..2945405	-	591	WP_223603888.1	malonic semialdehyde reductase	-
KGP20_RS14520 (KGP20_14465)	2945415..2946215	-	801	WP_223603889.1	pyrimidine utilization protein D	-
KGP20_RS14525 (KGP20_14470)	2946223..2946609	-	387	WP_223603890.1	pyrimidine utilization protein C	-
KGP20_RS14530 (KGP20_14475)	2946621..2947310	-	690	WP_047742244.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18951.65 Da
• Isoelectric Point: 7.9145

>T201768 WP_196350389.1 NZ_CP074177:2942393-2942893 [Enterobacter asburiae]
MLSEGTDYDFEDFDCGEPSLNAFLAEHLVRQHNGRILRAYLLKERDRPRVLGYYTLSGSCFERAMLPSKTQQRRIPYINV
PSVTLGRLAVDKPLQGNEWGTTLVAHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKQGFIPLTGENSHSLFFPTKSIE
RLFEQE

Nucleotide

• Length: 501 bp

>T201768 NZ_CP098754:c940014-939781 [Brachyspira pilosicoli]
ATGAGTAAGCTAATTTTGAAGGACGCAAAAACTATAGAAAAATTGTTGTTTTTATTAGGGTTTGTTAAACAGAGGCAGAA
AGGAAGTCATGCCTTTTATAAACATGAGGACGGACGATGCACTGTTATACCTTTTCACTCAAGCAAGGTAATATCAAGAC
CGCTTATGAGAAAAATATTAAATGAAATAAATCTTGATGTAGAAGAATATAATAATCTTATAGAAAAAATTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10262.72 Da
• Isoelectric Point: 5.8836

>AT201768 WP_008499829.1 NZ_CP074177:2942096-2942365 [Enterobacter asburiae]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT201768 NZ_CP098754:c940232-940011 [Brachyspira pilosicoli]
ATGAAGAAAAAGTTTTTTGCATATATAGAATATGATAAAGAAACCAATGTTTATGTGGGATATATTCCTTCAATAGTTGG
GGCACATACACAGGCTGACACTTTGGAAGAGTTGCATATAAAATTAAAAGAGGTTTTGGATTTATGTCTTGAAGAGATGG
ACAGCGAAGAAAAGAAGACTATTCCAGAGTTTGTAAAAGTAGAAGAGATAGAGCTTGTATGA

Structures

Antitoxin

• AlphaFold DB: W7PG43