Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2366742..2367362 Replicon chromosome
Accession NZ_CP074177
Organism Enterobacter asburiae strain JBIWA002

Toxin (Protein)


Gene name Hha Uniprot ID A0A1H8SI38
Locus tag KGP20_RS11780 Protein ID WP_008499287.1
Coordinates 2366742..2366960 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag KGP20_RS11785 Protein ID WP_008499288.1
Coordinates 2366988..2367362 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGP20_RS11750 (KGP20_11695) 2362756..2363016 + 261 WP_045356432.1 type B 50S ribosomal protein L31 -
KGP20_RS11755 (KGP20_11700) 2363019..2363159 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
KGP20_RS11760 (KGP20_11705) 2363156..2363866 - 711 WP_223605258.1 GNAT family protein -
KGP20_RS11765 (KGP20_11710) 2363968..2365428 + 1461 WP_223605259.1 PLP-dependent aminotransferase family protein -
KGP20_RS11770 (KGP20_11715) 2365400..2365867 - 468 WP_196350957.1 YlaC family protein -
KGP20_RS11775 (KGP20_11720) 2365985..2366536 - 552 WP_023310599.1 maltose O-acetyltransferase -
KGP20_RS11780 (KGP20_11725) 2366742..2366960 - 219 WP_008499287.1 HHA domain-containing protein Toxin
KGP20_RS11785 (KGP20_11730) 2366988..2367362 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
KGP20_RS11790 (KGP20_11735) 2367874..2371020 - 3147 WP_023310600.1 multidrug efflux RND transporter permease subunit AcrB -
KGP20_RS11795 (KGP20_11740) 2371043..2372236 - 1194 WP_023310601.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T201767 WP_008499287.1 NZ_CP074177:c2366960-2366742 [Enterobacter asburiae]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T201767 NZ_CP098753:c940015-939782 [Brachyspira pilosicoli]
ATGAGTAAGCTAATTTTGAAGGACGCAAAAACTATAGAAAAATTGTTGTTTTTATTAGGGTTTGTTAAACAGAGGCAGAA
AGGAAGTCATGCCTTTTATAAACATGAGGACGGACGATGCACTGTTATACCTTTTCACTCAAGCAAGGTAATATCAAGAC
CGCTTATGAGAAAAATATTAAATGAAATAAATCTTGATGTAGAAGAATATAATAATCTTATAGAAAAAATTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT201767 WP_008499288.1 NZ_CP074177:c2367362-2366988 [Enterobacter asburiae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT201767 NZ_CP098753:c940233-940012 [Brachyspira pilosicoli]
ATGAAGAAAAAGTTTTTTGCATATATAGAATATGATAAAGAAACCAATGTTTATGTGGGATATATTCCTTCAATAGTTGG
GGCACATACACAGGCTGACACTTTGGAAGAGTTGCATATAAAATTAAAAGAGGTTTTGGATTTATGTCTTGAAGAGATGG
ACAGCGAAGAAAAGAAGACTATTCCAGAGTTTGTAAAAGTAGAAGAGATAGAGCTTGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8SI38

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References