Detailed information of TA system TA201766

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1810469..1810985
• Replicon: chromosome
• Accession: NZ_CP074177
• Organism: Enterobacter asburiae strain JBIWA002

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KGP20_RS09170
• Protein ID: WP_045402335.1
• Coordinates: 1810701..1810985 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V3EXX3
• Locus tag: KGP20_RS09165
• Protein ID: WP_008501400.1
• Coordinates: 1810469..1810711 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP20_RS09150 (KGP20_09110)	1806467..1807207	+	741	WP_023310186.1	KDGP aldolase family protein	-
KGP20_RS09155 (KGP20_09115)	1807322..1808458	+	1137	WP_196351203.1	lactonase family protein	-
KGP20_RS09160 (KGP20_09120)	1808481..1810391	+	1911	WP_023310188.1	PRD domain-containing protein	-
KGP20_RS09165 (KGP20_09125)	1810469..1810711	+	243	WP_008501400.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
KGP20_RS09170 (KGP20_09130)	1810701..1810985	+	285	WP_045402335.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KGP20_RS09175 (KGP20_09135)	1810989..1811453	-	465	WP_223605100.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
KGP20_RS09180 (KGP20_09140)	1811613..1813751	-	2139	WP_023310191.1	anaerobic ribonucleoside-triphosphate reductase	-
KGP20_RS09185 (KGP20_09145)	1814128..1815771	-	1644	WP_223605101.1	alpha,alpha-phosphotrehalase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10907.73 Da
• Isoelectric Point: 10.3350

>T201766 WP_045402335.1 NZ_CP074177:1810701-1810985 [Enterobacter asburiae]
MTYELAFDPRALKEWSKLGQTVKDQFKKKLADVLVNPRVESARLHGLPDCYKIKLRTQGYRLVYQVQDNVVTVFVIAIGK
REKSAVYHDANKRL

Nucleotide

• Length: 285 bp

>T201766 NZ_CP098752:c940015-939782 [Brachyspira pilosicoli]
ATGAGTAAGCTAATTTTGAAGGACGCAAAAACTATAGAAAAATTGTTGTTTTTATTAGGGTTTGTTAAACAGAGGCAGAA
AGGAAGTCATGCCTTTTATAAACATGAGGACGGACGATGCACTGTTATACCTTTTCACTCAAGCAAGGTAATATCAAGAC
CGCTTATGAGAAAAATATTAAATGAAATAAATCTTGATGTAGAAGAATATAATAATCTTATAGAAAAAATTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8972.37 Da
• Isoelectric Point: 5.0651

>AT201766 WP_008501400.1 NZ_CP074177:1810469-1810711 [Enterobacter asburiae]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTISDSEDALIQTVRERLANPQKGIKVSLDDL

Nucleotide

• Length: 243 bp

>AT201766 NZ_CP098752:c940233-940012 [Brachyspira pilosicoli]
ATGAAGAAAAAGTTTTTTGCATATATAGAATATGATAAAGAAACCAATGTTTATGTGGGATATATTCCTTCAATAGTTGG
GGCACATACACAGGCTGACACTTTGGAAGAGTTGCATATAAAATTAAAAGAGGTTTTGGATTTATGTCTTGAAGAGATGG
ACAGCGAAGAAAAGAAGACTATTCCAGAGTTTGTAAAAGTAGAAGAGATAGAGCTTGTATGA

Structures

Antitoxin

• AlphaFold DB: V3EXX3