Detailed information of TA system TA201764

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1238969..1239585
• Replicon: chromosome
• Accession: NZ_CP074177
• Organism: Enterobacter asburiae strain JBIWA002

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KGP20_RS06305
• Protein ID: WP_080298642.1
• Coordinates: 1239214..1239585 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1Z3N2D4
• Locus tag: KGP20_RS06300
• Protein ID: WP_047743309.1
• Coordinates: 1238969..1239211 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP20_RS06275 (KGP20_06235)	1234946..1235545	+	600	WP_223604928.1	glucose-1-phosphatase	-
KGP20_RS06280 (KGP20_06240)	1235539..1236420	+	882	WP_223604929.1	virulence factor BrkB family protein	-
KGP20_RS06285 (KGP20_06245)	1236417..1236854	+	438	WP_008501836.1	D-aminoacyl-tRNA deacylase	-
KGP20_RS06290 (KGP20_06250)	1236899..1237840	+	942	WP_023309755.1	fatty acid biosynthesis protein FabY	-
KGP20_RS06295 (KGP20_06255)	1237849..1238769	-	921	WP_047743308.1	alpha/beta hydrolase	-
KGP20_RS06300 (KGP20_06260)	1238969..1239211	+	243	WP_047743309.1	ribbon-helix-helix protein, CopG family	Antitoxin
KGP20_RS06305 (KGP20_06265)	1239214..1239585	+	372	WP_080298642.1	type II toxin-antitoxin system VapC family toxin	Toxin
KGP20_RS06310 (KGP20_06270)	1239625..1240554	-	930	WP_010436850.1	formate dehydrogenase accessory protein FdhE	-
KGP20_RS06315 (KGP20_06275)	1240551..1241186	-	636	WP_023309750.1	formate dehydrogenase cytochrome b556 subunit	-
KGP20_RS06320 (KGP20_06280)	1241183..1242085	-	903	WP_008501831.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-109)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13688.85 Da
• Isoelectric Point: 6.9789

>T201764 WP_080298642.1 NZ_CP074177:1239214-1239585 [Enterobacter asburiae]
MEHMAVFDTNILIDLFNNRVEAADAIDRTASHRAISLITWMEVMVGARKHGHEAKTATVMGVFEIIDITRDIAERSVILR
EKHGMKLPDAIILATAQSRNCPLISRNTKDFSGITGVVSPYQL

Nucleotide

• Length: 372 bp

>T201764 NZ_CP098750:c969945-969712 [Brachyspira pilosicoli]
ATGAGTAAGCTAATTTTGAAGGACGCAAAAACTATAGAAAAATTGTTGTTTTTATTAGGGTTTGTTAAACAGAGGCAGAA
AGGAAGTCATGCCTTTTATAAACATGAGGACGGACGATGCACTGTTATACCTTTTCACTCAAGCAAGGTAATATCAAGAC
CGCTTATGAGAAAAATATTAAATGAAATAAATCTTGATGTAGAAGAATATAATAATCTTATAGAAAAAATTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9443.59 Da
• Isoelectric Point: 4.2917

>AT201764 WP_047743309.1 NZ_CP074177:1238969-1239211 [Enterobacter asburiae]
MSLSADLDMGRILIDLSDDVIQRLENLKQLRNRPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT201764 NZ_CP098750:c970163-969942 [Brachyspira pilosicoli]
ATGAAGAAAAAGTTTTTTGCATATATAGAATATGATAAAGAAACCAATGTTTATGTGGGATATATTCCTTCAATAGTTGG
GGCACATACACAGGCTGACACTTTGGAAGAGTTGCATATAAAATTAAAAGAGGTTTTGGATTTATGTCTTGAAGAGATGG
ACAGCGAAGAAAAGAAGACTATTCCAGAGTTTGTAAAAGTAGAAGAGATAGAGCTTGTATGA

Structures

Antitoxin

• AlphaFold DB: A0A1Z3N2D4