Detailed information of TA system TA201762

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 490227..491024
• Replicon: chromosome
• Accession: NZ_CP074177
• Organism: Enterobacter asburiae strain JBIWA002

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: KGP20_RS02680
• Protein ID: WP_223604733.1
• Coordinates: 490227..490616 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: KGP20_RS02685
• Protein ID: WP_147882818.1
• Coordinates: 490668..491024 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP20_RS02650 (KGP20_02630)	485392..487239	+	1848	WP_223604731.1	RNA polymerase sigma factor RpoD	-
KGP20_RS02655 (KGP20_02635)	487772..488278	-	507	WP_023309229.1	G/U mismatch-specific DNA glycosylase	-
KGP20_RS02665 (KGP20_02645)	488582..489424	-	843	WP_223605279.1	DUF4942 domain-containing protein	-
KGP20_RS02670 (KGP20_02650)	489507..489710	-	204	WP_212562729.1	hypothetical protein	-
KGP20_RS02675 (KGP20_02655)	489739..490230	-	492	WP_223604732.1	DUF5983 family protein	-
KGP20_RS02680 (KGP20_02660)	490227..490616	-	390	WP_223604733.1	TA system toxin CbtA family protein	Toxin
KGP20_RS02685 (KGP20_02665)	490668..491024	-	357	WP_147882818.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KGP20_RS02690 (KGP20_02670)	491075..491719	-	645	WP_223604734.1	antitoxin of toxin-antitoxin stability system	-
KGP20_RS02695 (KGP20_02675)	491735..491956	-	222	WP_042894348.1	DUF987 domain-containing protein	-
KGP20_RS02700 (KGP20_02680)	491972..492451	-	480	WP_223604735.1	DNA repair protein RadC	-
KGP20_RS02705 (KGP20_02685)	492463..492948	-	486	WP_223604736.1	antirestriction protein	-
KGP20_RS02710 (KGP20_02690)	493219..494037	-	819	WP_115117168.1	DUF932 domain-containing protein	-
KGP20_RS02715 (KGP20_02695)	494127..494375	-	249	WP_223604737.1	DUF905 domain-containing protein	-
KGP20_RS02720 (KGP20_02700)	494460..495410	-	951	WP_223604738.1	Ivy family c-type lysozyme inhibitor	-
KGP20_RS02725 (KGP20_02705)	495459..495674	-	216	WP_223604739.1	transposase	-
KGP20_RS02730 (KGP20_02710)	495686..495835	-	150	WP_223604740.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (18-116)

Antitoxin

• Antitoxin CbeA (15-113)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14656.77 Da
• Isoelectric Point: 8.8833

>T201762 WP_223604733.1 NZ_CP074177:c490616-490227 [Enterobacter asburiae]
MRIYPVSAKRAAEIELLTHKSPISVWQRLLSHLLDRHYGLTLNDTPFGNDDVIQEHIEAGISLCDAVNFIVEKYDLVRTV
RRGFSAETQSPLLTSIDILRARKATGLMTRNGYRAVTDITTGKYREVQQ

Nucleotide

• Length: 390 bp

>T201762 NZ_CP098748:c939867-939634 [Brachyspira pilosicoli]
ATGAGTAAGCTAATTTTGAAGGACGCAAAAACTATAGAAAAATTGTTGTTTTTATTAGGGTTTGTTAAACAGAGGCAGAA
AGGAAGTCATGCCTTTTATAAACATGAGGACGGACGATGCACTGTTATACCTTTTCACTCAAGCAAGGTAATATCAAGAC
CGCTTATGAGAAAAATATTAAATGAAATAAATCTTGATGTAGAAGAATATAATAATCTTATAGAAAAAATTTAA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13140.90 Da
• Isoelectric Point: 6.6322

>AT201762 WP_147882818.1 NZ_CP074177:c491024-490668 [Enterobacter asburiae]
MPDFPATNDNMDAPRWGLQRNITPCFGARLVQEGNRLHYLNDRASITGTFSNADLRHLDQAFPLLLKQLELMLVSGELNP
RHQHSITLYAKGLTCEADSLASHGYVYIAIYPTPATTA

Nucleotide

• Length: 357 bp

>AT201762 NZ_CP098748:c940085-939864 [Brachyspira pilosicoli]
ATGAAGAAAAAGTTTTTTGCATATATAGAATATGATAAAGAAACCAATGTTTATGTGGGATATATTCCTTCAATAGTTGG
GGCACATACACAGGCTGACACTTTGGAAGAGTTGCATATAAAATTAAAAGAGGTTTTGGATTTATGTCTTGAAGAGATGG
ACAGCGAAGAAAAGAAGACTATTCCAGAGTTTGTAAAAGTAGAAGAGATAGAGCTTGTATGA