Detailed information of TA system TA201761

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 398496..399295
• Replicon: chromosome
• Accession: NZ_CP074177
• Organism: Enterobacter asburiae strain JBIWA002

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: KGP20_RS02225
• Protein ID: WP_123060711.1
• Coordinates: 398918..399295 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: KGP20_RS02220
• Protein ID: WP_123060710.1
• Coordinates: 398496..398861 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP20_RS02175 (KGP20_02155)	393901..394308	+	408	WP_032233521.1	hypothetical protein	-
KGP20_RS02180 (KGP20_02160)	394380..394607	+	228	WP_123060703.1	hypothetical protein	-
KGP20_RS02185 (KGP20_02165)	394910..395746	+	837	WP_123060705.1	hypothetical protein	-
KGP20_RS02190 (KGP20_02170)	395840..396073	+	234	WP_123060706.1	DUF905 domain-containing protein	-
KGP20_RS02195 (KGP20_02175)	396177..396995	+	819	WP_143345534.1	DUF932 domain-containing protein	-
KGP20_RS02200 (KGP20_02180)	396995..397249	+	255	WP_223604708.1	hypothetical protein	-
KGP20_RS02205 (KGP20_02185)	397337..397747	+	411	Protein_362	antirestriction protein	-
KGP20_RS02210 (KGP20_02190)	397759..398223	+	465	WP_123060708.1	DNA repair protein RadC	-
KGP20_RS02215 (KGP20_02195)	398252..398473	+	222	WP_123060709.1	DUF987 domain-containing protein	-
KGP20_RS02220 (KGP20_02200)	398496..398861	+	366	WP_123060710.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KGP20_RS02225 (KGP20_02205)	398918..399295	+	378	WP_123060711.1	TA system toxin CbtA family protein	Toxin
KGP20_RS02230 (KGP20_02210)	399292..399744	+	453	WP_143345536.1	DUF5983 family protein	-
KGP20_RS02235 (KGP20_02215)	399813..400016	+	204	WP_123060713.1	DUF957 domain-containing protein	-
KGP20_RS02240 (KGP20_02220)	400098..400940	+	843	WP_123060714.1	DUF4942 domain-containing protein	-
KGP20_RS02245 (KGP20_02225)	401213..401626	-	414	WP_223604709.1	hypothetical protein	-
KGP20_RS02250 (KGP20_02230)	401888..402415	-	528	WP_223605277.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13754.84 Da
• Isoelectric Point: 9.9983

>T201761 WP_123060711.1 NZ_CP074177:398918-399295 [Enterobacter asburiae]
MQTLSAIPARKAPSHPTPVEIWQRLLTYLLERHYGLSLNDTQFGNDGVIQQHIDAGLSLADALNFLVEKSELVRIDRPGF
SIQHQSPFISAIDILRARKAIGLMQRTGYKAVTCAISGRSSGGLK

Nucleotide

• Length: 378 bp

>T201761 NZ_CP098746:18884-19030 [Enterococcus faecalis]
ATGAACGTACTTCCAAAATCTATAGAAAGGAGAAGCCTTTTGTCCGTTTCGGAAAGTTTGCAACTAATGCTTGCGTTTGG
TGGGTTTACATTGACCCTAATCACAACCATTGTTGCCATTCTGAACTATAAAGACAAAAAGAAATAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13398.24 Da
• Isoelectric Point: 5.9501

>AT201761 WP_123060710.1 NZ_CP074177:398496-398861 [Enterobacter asburiae]
MPNTTPVANHDISEPWWGLKPTVTPCFGARLVQEGNHLHYLADRSTIEGTFSDADLRHLDQAFPVLMKQLELMLVSGELN
PRNQHCVTLYAKGLTLDADTLGSCGYLYIAIYPTSGNSIPR

Nucleotide

• Length: 366 bp

>AT201761 NZ_CP098746:c19220-18964 [Enterococcus faecalis]
CACCGCCACCGCCGTTTAGGCGCGCATGAAAAAAGGTAGGCATTCGCCTACCTTTTCGCACAAAATGTGGTATAATAATT
ATGTAAAGAGGACGTACTGGTAATACGCCCTCATTGATAGCACCGCTAAAGACGGTGGCAGAGTTTTTATAAGTTATTTA
GAAATAACCGGTCTCCTGCTAAAGTGACGGTTATTTCTTTTTGTCTTTATAGTTCAGAATGGCAACAATGGTTGTGATTA
GGGTCAATGTAAACCCA