Detailed information of TA system TA020176

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 65075..65715
• Replicon: chromosome
• Accession: NC_000917
• Organism: Archaeoglobus fulgidus DSM 4304

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AF_RS00305
• Protein ID: WP_010877579.1
• Coordinates: 65075..65488 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A075W906
• Locus tag: AF_RS12305
• Protein ID: WP_052270455.1
• Coordinates: 65485..65715 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AF_RS13345 (AF_0054)	60485..60775	-	291	WP_244372781.1	hypothetical protein	-
AF_RS00260 (AF0055)	60772..61170	-	399	WP_010877569.1	DUF126 domain-containing protein	-
AF_RS00265 (AF0056)	61191..61331	-	141	WP_010877570.1	DNA-directed RNA polymerase subunit P	-
AF_RS00270 (AF0057)	61335..61586	-	252	WP_010877571.1	50S ribosomal protein L37Ae	-
AF_RS00275 (AF0058)	61625..62149	-	525	WP_010877572.1	PUA domain-containing protein	-
AF_RS00280 (AF0059)	62116..62520	-	405	WP_010877573.1	hypothetical protein	-
AF_RS00285 (AF0060)	62618..62899	+	282	WP_010877574.1	MazG nucleotide pyrophosphohydrolase domain-containing protein	-
AF_RS00290 (AF0061)	62899..63489	+	591	WP_010877575.1	dTMP kinase	-
AF_RS00295 (AF0062)	63463..64302	-	840	WP_048064160.1	CBS domain-containing protein	-
AF_RS12510	64406..64699	-	294	Protein_62	hypothetical protein	-
AF_RS12970	64826..64981	-	156	WP_156029468.1	hypothetical protein	-
AF_RS00305 (AF0065)	65075..65488	-	414	WP_010877579.1	type II toxin-antitoxin system VapC family toxin	Toxin
AF_RS12305 (AF_0066)	65485..65715	-	231	WP_052270455.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AF_RS00315 (AF0067)	65799..66485	-	687	WP_010877581.1	CRISPR-associated protein Cas5	-
AF_RS00320 (AF0068)	66497..67450	-	954	WP_010877582.1	DevR family CRISPR-associated autoregulator	-
AF_RS00325 (AF0069)	67451..67813	-	363	WP_010877583.1	hypothetical protein	-
AF_RS00330 (AF0070)	67800..68852	-	1053	WP_231487532.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-126)

Antitoxin

• SpoVT-AbrB domain (6-45)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 16415.97 Da
• Isoelectric Point: 4.5216

>T20176 WP_010877579.1 NC_000917:c65488-65075 [Archaeoglobus fulgidus DSM 4304]
MKIFLDANYLIYLKYSESDEIFDYCVNLLRKIEKYDLVTNMLAIDEVIWILNRKYKIELDEVFEYLDRLLSFLRVVPIEA
EDYDLMKEFMLGYNLKPSDSLHLSSMRRHGVSVIVSEDSDFDRVDWVKRVWIGRGDV

Nucleotide

• Length: 414 bp

>T20176 NC_000917:c65488-65075 [Archaeoglobus fulgidus DSM 4304]
ATGAAAATTTTTCTGGATGCCAATTATCTGATTTACCTGAAGTACTCTGAAAGTGACGAAATCTTTGATTACTGCGTAAA
CCTTCTCAGAAAAATTGAAAAATACGACCTGGTGACTAACATGCTCGCAATCGATGAGGTTATCTGGATACTCAACAGAA
AGTATAAAATCGAGCTTGATGAGGTGTTTGAGTATCTTGACAGACTTTTAAGCTTTCTCAGGGTTGTTCCGATTGAAGCT
GAGGATTACGACCTCATGAAGGAGTTCATGCTTGGATACAACCTCAAACCCTCGGACTCTCTGCATCTTTCGAGCATGAG
GAGACATGGGGTGAGTGTGATAGTCTCGGAGGATTCCGACTTTGACAGGGTGGATTGGGTTAAGAGGGTGTGGATTGGCA
GAGGTGATGTTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8649.22 Da
• Isoelectric Point: 6.3756

>AT20176 WP_052270455.1 NC_000917:c65715-65485 [Archaeoglobus fulgidus DSM 4304]
MVKLKVKVGAKGQVVIPKVIRDKLGIKPDDVLLVDEEGGKIVIEKQDIDDFIEWVKKTRKKVAGEVYRIALEDEFE

Nucleotide

• Length: 231 bp

>AT20176 NC_000917:c65715-65485 [Archaeoglobus fulgidus DSM 4304]
ATGGTAAAACTCAAGGTTAAAGTAGGTGCGAAGGGGCAGGTCGTGATTCCAAAGGTTATAAGGGACAAGCTGGGAATAAA
GCCAGATGATGTCCTTCTTGTGGATGAAGAGGGCGGGAAGATAGTGATTGAAAAGCAGGACATCGATGATTTCATTGAGT
GGGTCAAAAAAACCAGAAAGAAGGTTGCGGGCGAGGTTTACAGAATTGCATTGGAGGATGAGTTCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A075W906