Detailed information of TA system TA201757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 63339..64090
• Replicon: plasmid pJBIWA003_3
• Accession: NZ_CP074173
• Organism: Enterobacter sp. JBIWA003

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: KGP25_RS26585
• Protein ID: WP_223540748.1
• Coordinates: 63339..63821 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: KGP25_RS26590
• Protein ID: WP_223540749.1
• Coordinates: 63812..64090 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP25_RS26570 (KGP25_26425)	58898..61789	+	2892	WP_223540799.1	conjugal transfer mating-pair stabilization protein TraG	-
KGP25_RS26575 (KGP25_26430)	61804..62394	+	591	WP_223540800.1	protein traS	-
KGP25_RS26580 (KGP25_26435)	62564..63301	+	738	WP_223540801.1	conjugal transfer complement resistance protein TraT	-
KGP25_RS26585 (KGP25_26440)	63339..63821	-	483	WP_223540748.1	GNAT family N-acetyltransferase	Toxin
KGP25_RS26590 (KGP25_26445)	63812..64090	-	279	WP_223540749.1	DUF1778 domain-containing protein	Antitoxin
KGP25_RS26595 (KGP25_26450)	64228..66465	+	2238	WP_223540802.1	type IV conjugative transfer system coupling protein TraD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..75521	75521

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-91)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17378.95 Da
• Isoelectric Point: 8.4775

>T201757 WP_223540748.1 NZ_CP074173:c63821-63339 [Enterobacter sp. JBIWA003]
MGISAPEPLNSGHNIADFCCTEPGLNEWLKKKAHKNHSTGISRVYVICAENSHRVIGYYCLSTGSVQRNTAPGAYRRNAP
ESLPVIVLGRLAVDQAYSGQGLGVALLKDAVYRTEHIALQVGVKALVVHALSEEVSSFYTRFGFDASVVHPLTLLYPVKL

Nucleotide

• Length: 483 bp

>T201757 NZ_CP098743:2628397-2628540 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTTTTGTCAGCATATGAGACAATTCAGACGATTCTAGGTTTTGG
TATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10495.88 Da
• Isoelectric Point: 4.5141

>AT201757 WP_223540749.1 NZ_CP074173:c64090-63812 [Enterobacter sp. JBIWA003]
MKTEAREAPINIRARTSQRDLIDMAASLVSKSRTDFMLDAACREAQDILLDQRLFILDNAQYESFIAELDAPVTAERQAR
IDDLMGHKSPWE

Nucleotide

• Length: 279 bp

>AT201757 NZ_CP098743:c2628728-2628473 [Enterococcus faecalis]
ATAAAAATGCCCGAGCCTTGAAATAGGAGGCTCAGGCATTTTTTATTGATGGTCTTTTTAGGGTTGTTGTGCTATAATGA
TAAAGAAAAGAGAGATATGCTTCCAACATATCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCGTGTTAATTATTTAAA
AATAACCGTACTCAGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGCGCAATCAAAGCA
ATGGTAAACATACCAA