Detailed information of TA system TA201756

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 104274..105025
• Replicon: plasmid pJBIWA003_2
• Accession: NZ_CP074172
• Organism: Enterobacter sp. JBIWA003

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: KGP25_RS26155
• Protein ID: WP_223540748.1
• Coordinates: 104274..104756 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: KGP25_RS26160
• Protein ID: WP_223540749.1
• Coordinates: 104747..105025 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP25_RS26125 (KGP25_25990)	99549..99977	+	429	WP_223540747.1	antirestriction protein	-
KGP25_RS26130	100181..101526	+	1346	WP_123060779.1	IS3 family transposase	-
KGP25_RS26135 (KGP25_26005)	101573..102196	+	624	WP_143345831.1	IS66-like element accessory protein TnpA	-
KGP25_RS26140 (KGP25_26010)	102193..102540	+	348	WP_143345776.1	IS66 family insertion sequence element accessory protein TnpB	-
KGP25_RS26145 (KGP25_26015)	102560..104131	+	1572	WP_223540620.1	IS66 family transposase	-
KGP25_RS26155 (KGP25_26025)	104274..104756	-	483	WP_223540748.1	GNAT family N-acetyltransferase	Toxin
KGP25_RS26160 (KGP25_26030)	104747..105025	-	279	WP_223540749.1	DUF1778 domain-containing protein	Antitoxin
KGP25_RS26165 (KGP25_26035)	105163..107400	+	2238	WP_223540750.1	type IV conjugative transfer system coupling protein TraD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..116363	116363

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-91)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17378.95 Da
• Isoelectric Point: 8.4775

>T201756 WP_223540748.1 NZ_CP074172:c104756-104274 [Enterobacter sp. JBIWA003]
MGISAPEPLNSGHNIADFCCTEPGLNEWLKKKAHKNHSTGISRVYVICAENSHRVIGYYCLSTGSVQRNTAPGAYRRNAP
ESLPVIVLGRLAVDQAYSGQGLGVALLKDAVYRTEHIALQVGVKALVVHALSEEVSSFYTRFGFDASVVHPLTLLYPVKL

Nucleotide

• Length: 483 bp

>T201756 NZ_CP098743:c2623040-2622699 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCATGGGCAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10495.88 Da
• Isoelectric Point: 4.5141

>AT201756 WP_223540749.1 NZ_CP074172:c105025-104747 [Enterobacter sp. JBIWA003]
MKTEAREAPINIRARTSQRDLIDMAASLVSKSRTDFMLDAACREAQDILLDQRLFILDNAQYESFIAELDAPVTAERQAR
IDDLMGHKSPWE

Nucleotide

• Length: 279 bp

>AT201756 NZ_CP098743:c2623270-2623040 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAACTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGATGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA