Detailed information of TA system TA201755

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 84898..85433
• Replicon: plasmid pJBIWA003_2
• Accession: NZ_CP074172
• Organism: Enterobacter sp. JBIWA003

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KGP25_RS26025
• Protein ID: WP_223540737.1
• Coordinates: 84898..85185 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KGP25_RS26030
• Protein ID: WP_172686957.1
• Coordinates: 85182..85433 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP25_RS25995 (KGP25_25860)	80807..81505	-	699	WP_223540731.1	type VI secretion system protein TssL, short form	-
KGP25_RS26000 (KGP25_25865)	81794..82426	-	633	WP_223540732.1	recombinase family protein	-
KGP25_RS26005 (KGP25_25870)	82831..83166	-	336	WP_223540733.1	hypothetical protein	-
KGP25_RS26010 (KGP25_25875)	83518..83805	-	288	WP_223540734.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KGP25_RS26015 (KGP25_25880)	83802..84053	-	252	WP_223540735.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
KGP25_RS26020 (KGP25_25885)	84225..84542	-	318	WP_223540736.1	hypothetical protein	-
KGP25_RS26025 (KGP25_25890)	84898..85185	-	288	WP_223540737.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KGP25_RS26030 (KGP25_25895)	85182..85433	-	252	WP_172686957.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KGP25_RS26035 (KGP25_25900)	85793..85984	-	192	WP_176454398.1	hypothetical protein	-
KGP25_RS26040 (KGP25_25905)	86092..86394	-	303	WP_181244197.1	hypothetical protein	-
KGP25_RS26045 (KGP25_25910)	86391..87017	-	627	WP_223540738.1	ParA family plasmid-partitioning AAA ATPase	-
KGP25_RS26050 (KGP25_25915)	87187..88461	-	1275	WP_223540739.1	Y-family DNA polymerase	-
KGP25_RS26055 (KGP25_25920)	88461..88538	-	78	Protein_81	peptidase	-
KGP25_RS26060	88601..89113	-	513	Protein_82	transposase	-
KGP25_RS26065 (KGP25_25930)	89129..90106	+	978	WP_245405062.1	IS21 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..116363	116363

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11142.11 Da
• Isoelectric Point: 10.4701

>T201755 WP_223540737.1 NZ_CP074172:c85185-84898 [Enterobacter sp. JBIWA003]
MTYTVKFRDDALKEWQKLDKPIQQQFAKKLKKCSENPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDDQLIIAVVAVGK
RERSEVYNLASERMR

Nucleotide

• Length: 288 bp

>T201755 NZ_CP098743:c2554428-2554285 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTGTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAATAAAAAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9130.34 Da
• Isoelectric Point: 4.0780

>AT201755 WP_172686957.1 NZ_CP074172:c85433-85182 [Enterobacter sp. JBIWA003]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSSQTLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT201755 NZ_CP098743:2554166-2554352 [Enterococcus faecalis]
TGTGCTATAATGAAAACGAAAAGAGAGAGATGCGTCAACATACCTCTCTGATGTAGAACCGTTTAAGACGGTGACCGATT
TTGTTACAAAAAATAACCGTACTCGATCAAAGTAAACGGTTATTTTTTATTGTCATTTTTAAGCAATTTCACAATCAGCG
CAATCAAAGCAATGGTAAACATACCAA