Detailed information of TA system TA201733

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4894392..4894993
• Replicon: chromosome
• Accession: NZ_CP074168
• Organism: Serratia ureilytica strain JBIWA004

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KGP23_RS23320
• Protein ID: WP_147838417.1
• Coordinates: 4894613..4894993 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8B4GSF3
• Locus tag: KGP23_RS23315
• Protein ID: WP_025304767.1
• Coordinates: 4894392..4894613 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP23_RS23300 (KGP23_23240)	4890055..4891194	-	1140	WP_033645136.1	PLP-dependent aspartate aminotransferase family protein	-
KGP23_RS23305 (KGP23_23245)	4891274..4892053	-	780	WP_033645137.1	2OG-Fe dioxygenase family protein	-
KGP23_RS23310 (KGP23_23250)	4893068..4894318	+	1251	WP_047025992.1	valine--pyruvate transaminase	-
KGP23_RS23315 (KGP23_23255)	4894392..4894613	+	222	WP_025304767.1	ribbon-helix-helix domain-containing protein	Antitoxin
KGP23_RS23320 (KGP23_23260)	4894613..4894993	+	381	WP_147838417.1	type II toxin-antitoxin system VapC family toxin	Toxin
KGP23_RS23325 (KGP23_23265)	4895004..4896662	-	1659	WP_042783386.1	putative transporter	-
KGP23_RS23330 (KGP23_23270)	4896830..4897258	-	429	WP_004933922.1	small heat shock chaperone IbpB	-
KGP23_RS23335 (KGP23_23275)	4897365..4897778	-	414	WP_004933919.1	small heat shock chaperone IbpA	-
KGP23_RS23340 (KGP23_23280)	4898088..4898435	+	348	WP_016929593.1	YceK/YidQ family lipoprotein	-
KGP23_RS23345 (KGP23_23285)	4898466..4899746	-	1281	WP_197801817.1	DUF3748 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-100)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14271.68 Da
• Isoelectric Point: 7.3173

>T201733 WP_147838417.1 NZ_CP074168:4894613-4894993 [Serratia ureilytica]
MVAQRALFDTNILIDYLNGIPQARDVLVEYHINPAISAITWMEVMVGAKKQGPTLELKTRQFLGQFLLLPITDEVAERAV
ELRHSQHVKLPDAIIWATAQVGFRMLISRNPKDFGTDNGVLMPYRL

Nucleotide

• Length: 381 bp

>T201733 NZ_CP098738:c1443128-1442382 [Bacillus halotolerans]
ATGCTTTTATTCTTTCAAATCATGGTCTGGTGCACTATGGCCGCTTTAGGCTTATACGTATACGCCACATGGCGGTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGAAAAACATGGTATTTGCTGTTTGTTTTGGGCGCCATGATATACTGGA
CATATGATCCCTCTTCCCTGTTCACAAACTGGGAACGGTATGTCATTGTCGCTGTCAGCTTTGCTTTAATTGACGCTTTT
ATCTTTTTGAGCGCTTATGTGAAAAAACTGGCCGGCAGTGAACTGGAAACAGACACAAGAGAAATTCTCGAAGAAAACAA
CGAAATGCTCCACATGTATCTCGATCGGCTGAAAACATACCAATATCTATTGAAAAACGAACCGATTCATGTTTATTATG
GAAGTATAGAAGCATATGCTGAAGGAATTGATAAACTGCTGAAAACCTATGCTGATAAAATGAATCTGACGGCCAGCCTT
TGTTACTATTCGACACAGTCTGATAAAGACCGGTTAACCGAGCACATGGAGGATCCCGCTGACGTGCAAACGAGGCTGGA
TCGAAAGGATGTCTATTACGACCAGTATGGAAAAGTAGTGCTCATTCCCTTTACAATCGAGACACAGAGCTATGTCATCA
AACTGACGTCTGACAGCATTGTAACGGAATTTGATTATTTGCTATTCACATCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8607.55 Da
• Isoelectric Point: 4.4915

>AT201733 WP_025304767.1 NZ_CP074168:4894392-4894613 [Serratia ureilytica]
MTRIVIDLPEEDLRQLDNLKNIRHVPRAEIIRQAVSRYLTENHVEDPSNAFGLWSGAMGDGVEYENRQREEWE

Nucleotide

• Length: 222 bp

>AT201733 NZ_CP098738:c1442382-1442212 [Bacillus halotolerans]
ATGGAAAACGCGTTTCAAAACGGATGCCATCCGCGGGCAAAGAAACCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCACACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGATGAGTATAAACGTA
AAAAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A8B4GSF3