Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3846191..3846813 Replicon chromosome
Accession NZ_CP074168
Organism Serratia ureilytica strain JBIWA004

Toxin (Protein)


Gene name Hha Uniprot ID A0A8B4G7F9
Locus tag KGP23_RS18325 Protein ID WP_004940313.1
Coordinates 3846610..3846813 (+) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A2X2G5J9
Locus tag KGP23_RS18320 Protein ID WP_004940312.1
Coordinates 3846191..3846559 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGP23_RS18290 (KGP23_18230) 3842284..3842538 + 255 WP_004940303.1 type B 50S ribosomal protein L31 -
KGP23_RS18295 (KGP23_18235) 3842551..3842691 + 141 WP_004940304.1 type B 50S ribosomal protein L36 -
KGP23_RS18300 (KGP23_18240) 3842797..3843675 - 879 WP_042783804.1 zinc ABC transporter substrate-binding protein -
KGP23_RS18305 (KGP23_18245) 3843701..3844558 - 858 WP_033642105.1 metal ABC transporter permease -
KGP23_RS18310 (KGP23_18250) 3844555..3845268 - 714 WP_223524157.1 ABC transporter ATP-binding protein -
KGP23_RS18315 (KGP23_18255) 3845678..3846031 + 354 WP_047025751.1 hypothetical protein -
KGP23_RS18320 (KGP23_18260) 3846191..3846559 + 369 WP_004940312.1 Hha toxicity modulator TomB Antitoxin
KGP23_RS18325 (KGP23_18265) 3846610..3846813 + 204 WP_004940313.1 HHA domain-containing protein Toxin
KGP23_RS18330 (KGP23_18270) 3847095..3847613 - 519 WP_033651103.1 cytochrome b/b6 domain-containing protein -
KGP23_RS18335 (KGP23_18275) 3847666..3849093 - 1428 WP_223524160.1 N-acetylglucosamine-binding protein GbpA -
KGP23_RS18340 (KGP23_18280) 3849347..3849709 + 363 WP_033642100.1 type II secretion system pilot lipoprotein GspS -
KGP23_RS18345 (KGP23_18285) 3849706..3850467 - 762 WP_223525032.1 A24 family peptidase -
KGP23_RS18350 (KGP23_18290) 3850537..3851001 - 465 WP_223524162.1 type II secretion system protein GspM -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8107.37 Da        Isoelectric Point: 6.9756

>T201730 WP_004940313.1 NZ_CP074168:3846610-3846813 [Serratia ureilytica]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPTSVWKYVR

Download         Length: 204 bp

>T201730 NZ_CP098736:c983101-982820 [Cupriavidus gilardii]
ATGATCCAATCATTCCGATGCATGGACACGCGAGCCCTCTTTGAAGGCAAGCGCGTTGCACGTTTTACCAACATCCACGC
AGTTGCGGAACGCAAACTGCAGATGTTGGAGTCCGCCACCACACTGGCCTCGTTAATGGCTCCGCCAGGCAATCGGCTGG
AAATCCTCTCGGGCAATCGACGGGGCCAGTACAGCATCAGGGTGAACGCCCAATGGCGGATTTGTTTCCTCTGGACCTCC
GAAGGTCCACAGTGCGTAGAAATTGTGGACTACCATGATTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14261.95 Da        Isoelectric Point: 4.4314

>AT201730 WP_004940312.1 NZ_CP074168:3846191-3846559 [Serratia ureilytica]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEDICTTMKT

Download         Length: 369 bp

>AT201730 NZ_CP098736:c982827-982474 [Cupriavidus gilardii]
ATGATTGAGTTCAACAACAACGTTGGCGAGGAGTTCGCTATGGCGCGTTTTCACAACGGCATGCGCCCGGTTCATCCGGG
CGAGATCCTGCGCGAGGAGTATCTGGTGCCGCTCGATCTATCGATCGCGGAACTGGCGAAGGAATTGCATGTCGACACCG
CGCAGATCGACGCGATCGTCCGGGGCGATGCCGGCGTGGCCAGCGAGATCGCTCTGCGTTTGGCGCGCTATTTTGGTGGT
GACGCCGAAACCTGGTTGAACCTTCAGCAGGCCTACGATCTCAAGACCGCCGAACAGCAGATCGGCGACGCCATTCGCTC
CGAAGTCGCGCCGCGCAAGGCCAAGGCGGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4G7F9

Antitoxin

Source ID Structure
AlphaFold DB A0A2X2G5J9

References