Detailed information of TA system TA201717

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 505067..505662
• Replicon: chromosome
• Accession: NZ_CP074168
• Organism: Serratia ureilytica strain JBIWA004

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1S6LRI1
• Locus tag: KGP23_RS02355
• Protein ID: WP_071605322.1
• Coordinates: 505067..505321 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KGP23_RS02360
• Protein ID: WP_223294898.1
• Coordinates: 505318..505662 (+)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP23_RS02325 (KGP23_02320)	501370..501558	+	189	WP_223524486.1	hypothetical protein	-
KGP23_RS02330 (KGP23_02325)	501597..501734	+	138	Protein_451	DinI-like family protein	-
KGP23_RS02335 (KGP23_02330)	501860..502543	-	684	WP_223524489.1	hypothetical protein	-
KGP23_RS02340 (KGP23_02335)	502530..503609	-	1080	WP_223524492.1	hypothetical protein	-
KGP23_RS02345 (KGP23_02340)	503609..504610	-	1002	WP_223524495.1	hypothetical protein	-
KGP23_RS02350 (KGP23_02345)	504721..504939	+	219	WP_223524497.1	hypothetical protein	-
KGP23_RS02355 (KGP23_02350)	505067..505321	+	255	WP_071605322.1	type II toxin-antitoxin system HicA family toxin	Toxin
KGP23_RS02360 (KGP23_02355)	505318..505662	+	345	WP_223294898.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KGP23_RS02365 (KGP23_02360)	505707..506741	-	1035	WP_223524510.1	phage portal protein	-
KGP23_RS02370 (KGP23_02365)	506738..507424	-	687	WP_223524513.1	terminase family protein	-
KGP23_RS02375 (KGP23_02370)	507424..509196	-	1773	WP_033632041.1	terminase ATPase subunit family protein	-
KGP23_RS02380 (KGP23_02375)	509339..510154	+	816	WP_223524516.1	GPO family capsid scaffolding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	aac(6')-Ic	-	490679..540434	49755

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (56-101)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9495.97 Da
• Isoelectric Point: 11.1073

>T201717 WP_071605322.1 NZ_CP074168:505067-505321 [Serratia ureilytica]
MNKRHQKTLSDVFARPVNGSIKWSDIEALFTALGAEIHEREGSRIAVLLKGEKRVFHRPHPRPTTDKGAVNSIRIWLDSL
GIKP

Nucleotide

• Length: 255 bp

>T201717 NZ_CP098725:c60200-59877 [Yersinia ruckeri]
ATGGAGTATCTAGAATTCATTGAGACCCCCGTATTTAGCAGGCGAAGAGCTGAACTGCTGCCAGATGATGACTTCAGAGC
TTTACAGGAGCATCTTCTTAAAAACCATGATCTTGGCGACACAATCAGTAAAACAGGTGGATGTAAGAAAATCCGCTGGA
GTCGCGAAGGCATGGGGAAGCGGGGGGGAGTAAGAGTTATTTATTACGTCATAACCAAATCGGGACGCCTTTATCTGCTA
CTGGTCTATCCCAAAAACGAAAAAGACGATCTGACTGAAGCCGAAAAAGCTGTCCTGAAATCGATAAGTCAGCAAATCGA
ATAA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12531.10 Da
• Isoelectric Point: 4.5789

>AT201717 WP_223294898.1 NZ_CP074168:505318-505662 [Serratia ureilytica]
MMNNTLKIDGHTAVINFDPEIEMFRGEFVGLNGGADFYAYSVDELKKEGAISLAVFLDECHKDGIEPYKSYSGKVTTRLS
PERHQALAIAAQATGQSINELLNEGVDLVIEKHS

Nucleotide

• Length: 345 bp

>AT201717 NZ_CP098725:c59831-59535 [Yersinia ruckeri]
ATGAAAACTGAACTGTTTAATGATCTCGTTGAAAGTATGCAACAGATGGTTGCTATTGAGAAAGGTACGCTCTCTGTTCC
AGCGGAAAACATCCATCGTCATCGACTTCCAGATGTAAAATCAATGCGTGAAGCGAGCGGTCTTAAGCAAAATGAATTTG
CGGATGTAGTGGGGGTTTCTGCTTCTCTGCTCCAGAGCTGGGAACAGCATCGTCGTATTCCATCAGGTTCATCTCTAAAG
TTGTTGATGATGATAGAGCGTGACCCAGCTGTTATAGACGCTTTACGAACGCTTTAG

Structures

Toxin

• AlphaFold DB: A0A1S6LRI1