Detailed information of TA system TA201714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 80967..81718
• Replicon: plasmid pJBIWA005_2
• Accession: NZ_CP074161
• Organism: Enterobacter sp. JBIWA005

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: KGP22_RS23925
• Protein ID: WP_223540748.1
• Coordinates: 80967..81449 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: KGP22_RS23930
• Protein ID: WP_223540749.1
• Coordinates: 81440..81718 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP22_RS23895 (KGP22_23760)	76315..76578	+	264	WP_223582726.1	hypothetical protein	-
KGP22_RS23900 (KGP22_23765)	76620..77450	+	831	WP_223582727.1	N-6 DNA methylase	-
KGP22_RS23905 (KGP22_23770)	78074..78604	+	531	WP_223582728.1	antirestriction protein	-
KGP22_RS23910 (KGP22_23775)	78594..79070	-	477	WP_223540835.1	transglycosylase SLT domain-containing protein	-
KGP22_RS23920 (KGP22_23785)	80660..80929	+	270	Protein_103	complement resistance protein TraT	-
KGP22_RS23925 (KGP22_23790)	80967..81449	-	483	WP_223540748.1	GNAT family N-acetyltransferase	Toxin
KGP22_RS23930 (KGP22_23795)	81440..81718	-	279	WP_223540749.1	DUF1778 domain-containing protein	Antitoxin
KGP22_RS23935 (KGP22_23800)	81856..84093	+	2238	WP_223582732.1	type IV conjugative transfer system coupling protein TraD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..92286	92286

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-91)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17378.95 Da
• Isoelectric Point: 8.4775

>T201714 WP_223540748.1 NZ_CP074161:c81449-80967 [Enterobacter sp. JBIWA005]
MGISAPEPLNSGHNIADFCCTEPGLNEWLKKKAHKNHSTGISRVYVICAENSHRVIGYYCLSTGSVQRNTAPGAYRRNAP
ESLPVIVLGRLAVDQAYSGQGLGVALLKDAVYRTEHIALQVGVKALVVHALSEEVSSFYTRFGFDASVVHPLTLLYPVKL

Nucleotide

• Length: 483 bp

>T201714 NZ_CP098724:c2078628-2078535 [Yersinia ruckeri]
TAAGCCTGCATGAAATGCCAACTTTTAGCGCACGGCTCTATCCCAAGAGCCATTTCCCTGGACCGAATATAGGATTCGTA
TTCGGTCTTTTTTT

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10495.88 Da
• Isoelectric Point: 4.5141

>AT201714 WP_223540749.1 NZ_CP074161:c81718-81440 [Enterobacter sp. JBIWA005]
MKTEAREAPINIRARTSQRDLIDMAASLVSKSRTDFMLDAACREAQDILLDQRLFILDNAQYESFIAELDAPVTAERQAR
IDDLMGHKSPWE

Nucleotide

• Length: 279 bp

>AT201714 NZ_CP098724:2078531-2078677 [Yersinia ruckeri]
AGATAAAAAAAGACCGAATACGAATCCTATATTCGGTCCAGGGAAATGGCTCTTGGGATAGAGCCGTGCGCTAAAAGTTG
GCATTTCATGCAGGCTTATTAAGCCGTACCACTTAAGCGTAGTAGACGACCCACATTTTACCAATTT