Detailed information of TA system TA201712

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 57995..58632
• Replicon: plasmid pJBIWA005_2
• Accession: NZ_CP074161
• Organism: Enterobacter sp. JBIWA005

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: KGP22_RS23755
• Protein ID: WP_123061016.1
• Coordinates: 57995..58405 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: KGP22_RS23760
• Protein ID: WP_123061017.1
• Coordinates: 58402..58632 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP22_RS23725 (KGP22_23600)	54252..54575	+	324	WP_223582712.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KGP22_RS23730 (KGP22_23605)	54656..55684	+	1029	WP_223579877.1	IS21 family transposase	-
KGP22_RS23735 (KGP22_23610)	55681..56472	+	792	WP_223540741.1	IS21-like element helper ATPase IstB	-
KGP22_RS23745 (KGP22_23620)	57045..57668	+	624	WP_223540699.1	helix-turn-helix domain-containing protein	-
KGP22_RS23750 (KGP22_23625)	57734..57913	+	180	Protein_69	IS110 family transposase	-
KGP22_RS23755 (KGP22_23630)	57995..58405	-	411	WP_123061016.1	PIN domain-containing protein	Toxin
KGP22_RS23760 (KGP22_23635)	58402..58632	-	231	WP_123061017.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KGP22_RS23765 (KGP22_23640)	59247..59609	+	363	WP_223582714.1	hypothetical protein	-
KGP22_RS23770 (KGP22_23645)	59640..59969	+	330	WP_223582716.1	hypothetical protein	-
KGP22_RS23775 (KGP22_23650)	60017..60415	+	399	WP_223582718.1	hypothetical protein	-
KGP22_RS23780 (KGP22_23655)	60520..61299	+	780	WP_223582720.1	site-specific integrase	-
KGP22_RS23785 (KGP22_23660)	61699..62001	-	303	WP_172686959.1	hypothetical protein	-
KGP22_RS23790 (KGP22_23665)	62154..62471	-	318	WP_223540736.1	hypothetical protein	-
KGP22_RS23795 (KGP22_23670)	62827..63114	-	288	WP_223540737.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KGP22_RS23800 (KGP22_23675)	63111..63362	-	252	WP_172686957.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..92286	92286
	-	flank	IS/Tn	-	-	55681..56472	791

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-118)

Antitoxin

• SpoVT-AbrB domain (7-48)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14665.00 Da
• Isoelectric Point: 6.4606

>T201712 WP_123061016.1 NZ_CP074161:c58405-57995 [Enterobacter sp. JBIWA005]
VNKTYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITWSEISFAARAAGPVTQQLAEAFCARLDAILPWDRAAV
DATTEVKVALRLAGTPPGPNDTAIAGHAIAAGAVLVTRIGGEFERVPGLMLEDWVK

Nucleotide

• Length: 411 bp

>T201712 NZ_CP098724:c954535-954332 [Yersinia ruckeri]
ATGACCAAAACTGACTACCTAATGCGTTTAAGAAAATGCACAACAATTGACACACTAGAGCGTGTTATTGAAAAAAATAA
GTATGAGCTATCTGATGATGAACTAGAATTATTCTATTCTGCTGCGGATCATCGTCTAGCTGAACTTACCATGAATAAGT
TATACGATAAAATTCCTGCAACTGTCTGGCAACATGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8654.78 Da
• Isoelectric Point: 4.7571

>AT201712 WP_123061017.1 NZ_CP074161:c58632-58402 [Enterobacter sp. JBIWA005]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELVIVREGDSIILRPVRPTWGSFAQFEKADPDFMAERGDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT201712 NZ_CP098724:c954955-954587 [Yersinia ruckeri]
ATGGATGAGTACTCACCTAAGCGACATGATATTGCTCAGTTGAAATTCTTATGTGAGAATTTGTATGATGAAGGTATCGC
CACATTGGGCGATAGCCACCACGGCTGGGTTAATGATCCAACCTCCGCGGTAAATCTCCAACTGAATGATTTAATCGAAC
ACATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACGCAGACGAAATCGATCTCTGCGAGTTGGTGGAGGAATATCTA
GACGATACATATACCTTGTTCAGCAGTTACGGAATCAATGATCCTGATTTACGCCGCTGGCAGAAAACAAAAGACCGATT
ATTCAGACTATTTTCAGGGGAATACCTATGTGCCCTGATGAAAAATTAA