Detailed information of TA system TA201711

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 37381..37855
• Replicon: plasmid pJBIWA005_2
• Accession: NZ_CP074161
• Organism: Enterobacter sp. JBIWA005

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: KGP22_RS23620
• Protein ID: WP_004197762.1
• Coordinates: 37589..37855 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: KGP22_RS23615
• Protein ID: WP_000879771.1
• Coordinates: 37381..37605 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP22_RS23585 (KGP22_23460)	32962..34155	+	1194	WP_223582618.1	MFS transporter	-
KGP22_RS23590 (KGP22_23465)	34407..34753	-	347	Protein_39	transposase	-
KGP22_RS23595 (KGP22_23470)	34965..35774	-	810	WP_223582620.1	TSUP family transporter	-
KGP22_RS23600 (KGP22_23475)	35889..36104	-	216	Protein_41	transposase	-
KGP22_RS23610 (KGP22_23485)	36357..36773	-	417	WP_223582791.1	Exc2 family lipoprotein	-
KGP22_RS23615 (KGP22_23490)	37381..37605	+	225	WP_000879771.1	DUF6290 family protein	Antitoxin
KGP22_RS23620 (KGP22_23495)	37589..37855	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KGP22_RS23625 (KGP22_23500)	37931..38176	-	246	WP_223582793.1	transposase	-
KGP22_RS23630 (KGP22_23505)	38421..38576	-	156	WP_223582795.1	hypothetical protein	-
KGP22_RS23635 (KGP22_23510)	39454..39828	+	375	WP_223582796.1	hypothetical protein	-
KGP22_RS23640 (KGP22_23515)	39882..40229	+	348	WP_223582798.1	hypothetical protein	-
KGP22_RS23645 (KGP22_23520)	40398..41018	+	621	Protein_49	VENN motif pre-toxin domain-containing protein	-
KGP22_RS23650 (KGP22_23525)	41756..41998	+	243	Protein_50	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..92286	92286

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T201711 WP_004197762.1 NZ_CP074161:37589-37855 [Enterobacter sp. JBIWA005]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T201711 NZ_CP098724:457644-458300 [Yersinia ruckeri]
GTGGCCTATGACTACAGCACTAGCCTCAACCCGGAAAAAGCCTTGATCTGGCGCATTGTCCATCGGGACAATATTCCGTG
GATACTGGACAACGGTCTGCACTGTGGCAACAGTCCGGTGCAGGCGGAAGACTGGATTAATATCGGTAACCCGGAGCTTA
TCGACAAACGTGCCGGACATCTGGTGCCAACCGGAATGCGGGGGACGCTGCATGACTATGTGCCGTTTTACTTTACTCCT
TTCTCGCCGATGCTGATGAATATCCGCAGCGGACGTGGCGGCATTAAGCAACGCCCCAATGAGGAGATCGTTATTCTGGT
CAGTAGCCTGCGAAACATGGTGGCGCAAAATGTCTCCTTCGTGTTTACCGATAGCCACGCATATTATAACCTGGCTAATT
ACTTTACCGAGCTGGACAGTCTGAACCAGATCGATTGGCCCATTCTACAAGCGCGGGATTTCCGTCGCGATCAGGACGAT
TTGGCCAAGTTCGAACGCTATCAGGCAGAGGCGTTAATTTGGAAGCACTGCCCAATAGCGCTGCTTGGCGGGATGATTTG
TTACAACGATGGCGTCAGGTTACAATTAGAGCAGTGGTTGTCGCAACGCAATCTGTCAATGCCCGTTCACGCGCGGGCTG
GGTGGTACTTCTCATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT201711 WP_000879771.1 NZ_CP074161:37381-37605 [Enterobacter sp. JBIWA005]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT201711 NZ_CP098724:458297-459364 [Yersinia ruckeri]
ATGATTACATTTACACAAGGCAATTTACTGGATGCGCCCGTAGAAGCACTAGTGAATACGGTTAATACCGTCGGTGTCAT
GGGTAAAGGCATCGCGCTGATGTTCAAAGAGCGCTTCCCCGTCAATATGAAAGCCTATGCTCTCGCCTGCAAACATAAGC
AGGTAATGACAGGTAAAATGTTTATTACCGAAACCGGCGAACTGATGGGGCCTCGCTGGATTGTCAACTTCCCAACTAAA
CAACACTGGCGCGCAGACTCCCGCATGGAGTGGGTTGAAGACGGCTTGCAGGATTTACGCCGCTTTTTGATCGAAGAGAA
AGTGCAATCTATCGCCATTCCCCCACTGGGGGCGGGTAACGGCGGCCTGAATTGGTCGGACGTTCGGATGCGGATTGAAT
CTGCGCTGGGCGACCTTCAGGACACGGAAATTCTGATTTATGAGCCTACCGAAAAATACCAGAACGTCGCCAAGAGCACC
GGCGTAAAAAAGCTCACTCCCGCCAGAGCGCTGATTGCTGAACTGGTGCGCCGCTACTGGGTGCTGGGCATGGAGTGCAG
CCTGCTGGAAATCCAGAAGCTGGCATGGCTGTTACAACGCGCCATTGAACAGCATCAGCAGGAAGATGTGCTGAAACTGC
GGTTTGAGGCGCACTATTACGGCCCCTACGCCCCCAACCTTAACCATCTGCTTAACGCGCTAGATGGCACGTATCTGAAA
GCAGAGAAACGCATCCCGGACACCCAGCCGCTGGATGTGATCTGGTTTAATGATCAGGAAAAAGAACACATCAACCTCTG
GCTGAACAACGAGGCCAAAGAGTGGCTACCGGCGCTTGAGCAGGTCAGTCAGTTGATTGACGGCTTTGAATCGCCGTTTG
GTCTGGAGCTGCTGGCAACCGTTGACTGGCTGCTGACCCGAGGAAAATGCCAGCCCACGCTGGATTCCGTGAAAGAGGGG
CTGCACCAGTGGCCTGCGGGTGAACGCTGGGCCAGCCGTAAGATGAAGCTCTTTGACGACAAGAATCTGCAATTTGCCAT
CAACCGCGTGATGGAGTTCCATTGCTAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5