Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 123386..123911 Replicon plasmid pJBIWA005_1
Accession NZ_CP074160
Organism Enterobacter sp. JBIWA005

Toxin (Protein)


Gene name ccdB Uniprot ID -
Locus tag KGP22_RS22975 Protein ID WP_123061019.1
Coordinates 123606..123911 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID -
Locus tag KGP22_RS22970 Protein ID WP_123061018.1
Coordinates 123386..123604 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGP22_RS22940 (KGP22_22830) 119368..119523 + 156 Protein_119 transposase -
KGP22_RS22950 (KGP22_22840) 121172..121795 + 624 WP_223540699.1 helix-turn-helix domain-containing protein -
KGP22_RS22955 (KGP22_22845) 121861..122040 + 180 Protein_122 IS110 family transposase -
KGP22_RS22960 (KGP22_22850) 122122..122532 - 411 WP_123061016.1 PIN domain-containing protein -
KGP22_RS22965 (KGP22_22855) 122529..122759 - 231 WP_123061017.1 type II toxin-antitoxin system VapB family antitoxin -
KGP22_RS22970 (KGP22_22860) 123386..123604 + 219 WP_123061018.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KGP22_RS22975 (KGP22_22865) 123606..123911 + 306 WP_123061019.1 type II toxin-antitoxin system toxin CcdB Toxin
KGP22_RS22980 (KGP22_22870) 123999..124778 + 780 WP_123061020.1 site-specific integrase -
KGP22_RS22985 (KGP22_22875) 125220..125462 + 243 Protein_128 transposase -
KGP22_RS22990 (KGP22_22880) 125692..127032 + 1341 WP_223582563.1 IS110 family transposase -
KGP22_RS22995 127022..127156 + 135 Protein_130 IS3 family transposase -
KGP22_RS23000 (KGP22_22885) 127610..127990 + 381 WP_073001090.1 transposase -
KGP22_RS23005 (KGP22_22890) 127987..128334 + 348 WP_073001021.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaFRI-5 vipA/tssB / vipB/tssC / hcp/tssD / clpV/tssH 1..191037 191037
- inside IScluster/Tn - - 119657..136922 17265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11613.28 Da        Isoelectric Point: 6.4673

>T201709 WP_123061019.1 NZ_CP074160:123606-123911 [Enterobacter sp. JBIWA005]
MQFRVYTYKRESRYRLFVDVQSDIVDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTAAVTGEEV
ADLSHRENEIKNAINLMFWGV

Download         Length: 306 bp

>T201709 NZ_CP098723:c3142394-3142104 [Yersinia ruckeri]
ATGACCTTTAATATCGACTTTGACGAACGGGCATTAAAAGAATGGCACAAACTAGATAAAACTATCCGAGAGCAATTTAA
AAAGAAACTAAGAAAATTACAGGAAAATCCCTATATTGAGTCAGCACGTTTGCACGGTGATCTGGCTGGTTGTTTCAAAA
TAAAATTACGCGCATCTGGCTTTCGGTTGATTTACCAAGTTATCGATGAGGAGATTGTGATTTGGATCGTTGCTGTTGGT
AAACGGGAAGATGAAAAAGCTTATGATATTGCCAAGAAGCGAATCCAATAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8335.22 Da        Isoelectric Point: 4.7405

>AT201709 WP_123061018.1 NZ_CP074160:123386-123604 [Enterobacter sp. JBIWA005]
MKQRITVTIDSDSYRLLKSADVNISGLVNTAMQNEARRLRAERWQTENQEGMAEVARFIETNGSFADENRDW

Download         Length: 219 bp

>AT201709 NZ_CP098723:c3142638-3142384 [Yersinia ruckeri]
ATGAGTACTATCCAAATTCGTGTGGATGATGAACTGAAGAAAAGTGCATATCAGGCGTTTGAAAAACTGAATCTTTCGCC
TTCTGATGCACTACGCCTGTTCCTACGTTATGTTGCAGAGAACGAAAAGCTGCCTTTTTCAGAAGTATCCGTCATGGTCA
GTAATCAAGATGAGGATGCTGATATTTTGGCTGTTGTCCGTGAACGTTTGAAAAATCCTGCAAAGCGTATCAGAGTAAAT
ATTGATGACCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References