Detailed information of TA system TA020170

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 332578..334067
• Replicon: plasmid pNGR234a
• Accession: NC_000914
• Organism: Sinorhizobium fredii NGR234

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: -
• Locus tag: NGR_RS31190
• Protein ID: WP_010875279.1
• Coordinates: 332805..334067 (+)
• Length: 421 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: A0A249Q849
• Locus tag: NGR_RS31185
• Protein ID: WP_010875278.1
• Coordinates: 332578..332808 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGR_RS31165	328380..329381	+	1002	WP_010875274.1	ABC transporter permease	-
NGR_RS31170	329425..330399	+	975	WP_010875275.1	substrate-binding domain-containing protein	-
NGR_RS31175	330457..331350	+	894	WP_010875276.1	amidohydrolase	-
NGR_RS32660	331511..331872	-	362	Protein_298	GNAT family N-acetyltransferase	-
NGR_RS32665	331882..331996	-	115	Protein_299	DUF1778 domain-containing protein	-
NGR_RS31185	332578..332808	+	231	WP_010875278.1	helix-turn-helix transcriptional regulator	Antitoxin
NGR_RS31190	332805..334067	+	1263	WP_010875279.1	type II toxin-antitoxin system HipA family toxin	Toxin
NGR_RS31195	334778..335032	+	255	WP_165447175.1	hypothetical protein	-
NGR_RS31200	335076..335213	-	138	WP_158228604.1	hypothetical protein	-
NGR_RS31205	335573..336286	-	714	WP_010875281.1	hypothetical protein	-
NGR_RS31210	336285..336518	+	234	WP_164924747.1	hypothetical protein	-
NGR_RS31215	336958..337647	-	690	WP_010875282.1	OmpW family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd	1..536165	536165

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (151-386)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 421 a.a.
• Molecular weight: 45729.17 Da
• Isoelectric Point: 4.8411

>T20170 WP_010875279.1 NC_000914:332805-334067 [Sinorhizobium fredii NGR234]
MTVRVLNVWWDGRIVGQFTQDRHGDIGFAYSEAWLDDENTLPLSASLPKRAEPFSRRECRPFFGGLLPEESQRLVTAQAL
GVSPANDFALLDRLGGDVAGALQLLPEDQEPIEAGPLPDQQPTPLDEAGIVRILDALPTRPLLAGQEGLRLSLAGAQSKV
PLVLIDGELALPVSGQATTHILKPPIARFPGTTENEAFVMRLAAAIGLDVAPVEPRSANGRPFLVVERYDRYRDADGVVH
RIHQEDFCQALGVPPETKYASEGGPTFKDCFELLRRVSARPATDVLKLLDAAIFNLVVGNADAHGKNFSILYDDQGPKMA
PLYDLLSTVAYPDLSPKMAMRIGKRVTLAEMDADGWQTFAKEAGVGLPLVRRRITNLVDSTAEAVARVLEDTSDLYIDSA
RINHFADSVAGRAKLVRLSI

Nucleotide

• Length: 1263 bp

>T20170 NC_000914:332805-334067 [Sinorhizobium fredii NGR234]
ATGACGGTAAGGGTTCTCAACGTCTGGTGGGATGGTCGCATTGTCGGGCAATTCACCCAGGACAGGCACGGTGATATCGG
CTTTGCCTACAGCGAAGCTTGGCTCGACGACGAAAACACGCTTCCGCTGTCTGCCTCCCTCCCGAAGCGCGCAGAACCTT
TTTCTCGTCGTGAATGCCGACCATTTTTCGGCGGCCTGTTGCCCGAGGAAAGCCAGCGTCTTGTGACAGCGCAGGCATTG
GGCGTTTCACCTGCGAACGACTTTGCGCTCCTTGATCGCTTGGGCGGCGACGTCGCCGGCGCACTTCAGCTTTTGCCGGA
AGATCAGGAGCCCATAGAGGCAGGGCCGCTTCCAGACCAGCAGCCGACACCTCTGGATGAGGCCGGAATCGTTCGGATAC
TGGACGCACTTCCGACGCGGCCGCTATTGGCCGGCCAGGAAGGCTTGAGGCTATCGCTAGCCGGCGCGCAGTCCAAGGTG
CCGCTTGTTTTGATTGACGGCGAGCTCGCGCTTCCAGTTTCCGGACAAGCAACAACACATATTCTGAAACCACCAATCGC
GCGCTTTCCAGGGACAACCGAAAACGAAGCATTCGTGATGAGACTCGCTGCAGCGATCGGCCTCGACGTTGCACCAGTCG
AACCGAGGTCTGCGAATGGGCGCCCTTTTCTTGTGGTCGAGCGATACGATCGCTATCGCGATGCCGACGGTGTGGTTCAC
CGCATCCACCAGGAAGACTTTTGCCAAGCACTTGGTGTGCCGCCTGAAACGAAATACGCCAGCGAGGGCGGGCCGACATT
CAAGGACTGCTTTGAGCTGTTGCGGCGAGTATCAGCGCGGCCCGCTACAGATGTGTTGAAGCTGCTGGACGCCGCGATCT
TCAACCTGGTTGTCGGAAATGCCGATGCCCACGGCAAGAATTTCTCGATCCTCTACGACGATCAGGGACCGAAAATGGCC
CCGCTGTACGATCTGCTTTCTACGGTCGCCTATCCAGACCTTTCACCGAAAATGGCCATGAGGATCGGGAAACGGGTCAC
GCTTGCCGAGATGGACGCTGATGGGTGGCAGACTTTTGCAAAGGAAGCTGGAGTTGGCTTACCTTTGGTGCGCCGCCGAA
TTACAAACTTGGTCGACTCCACCGCTGAAGCCGTAGCGAGAGTATTGGAAGACACCTCAGACCTCTACATCGATTCAGCT
AGGATAAATCACTTTGCGGACTCGGTTGCAGGGCGCGCCAAACTCGTCAGGTTGTCGATTTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8204.72 Da
• Isoelectric Point: 10.7650

>AT20170 WP_010875278.1 NC_000914:332578-332808 [Sinorhizobium fredii NGR234]
MAKRIKTPADIGALVRIVRKEQNLRQDELAGVAGVGLRFIVDLEAGKPTAQIGKVLQVLQTLGCSIDILAPGERRK

Nucleotide

• Length: 231 bp

>AT20170 NC_000914:332578-332808 [Sinorhizobium fredii NGR234]
ATGGCGAAGCGCATCAAAACTCCAGCCGATATCGGCGCTCTAGTCCGCATCGTGCGCAAGGAGCAAAACTTGCGGCAGGA
TGAGCTTGCGGGCGTTGCCGGCGTTGGCCTCCGGTTCATTGTCGATCTCGAAGCGGGCAAACCGACAGCTCAGATAGGAA
AAGTGTTGCAGGTTTTGCAAACACTCGGCTGTTCGATCGATATTCTGGCTCCTGGTGAACGTAGAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A249Q849