Detailed information of TA system TA201694

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 539693..540492
• Replicon: chromosome
• Accession: NZ_CP074159
• Organism: Enterobacter sp. JBIWA005

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W3D2T8
• Locus tag: KGP22_RS02530
• Protein ID: WP_045405101.1
• Coordinates: 540115..540492 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: KGP22_RS02525
• Protein ID: WP_045405099.1
• Coordinates: 539693..540052 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP22_RS02485 (KGP22_02470)	535584..536255	+	672	WP_045405089.1	EcsC family protein	-
KGP22_RS02490 (KGP22_02475)	536252..536503	+	252	WP_023312599.1	DUF905 family protein	-
KGP22_RS02495 (KGP22_02480)	536619..537437	+	819	WP_045405091.1	DUF932 domain-containing protein	-
KGP22_RS02500 (KGP22_02485)	537437..537661	+	225	WP_045405093.1	hypothetical protein	-
KGP22_RS02505 (KGP22_02490)	537786..538265	+	480	WP_080461205.1	antirestriction protein	-
KGP22_RS02510 (KGP22_02495)	538276..538755	+	480	WP_045405095.1	DNA repair protein RadC	-
KGP22_RS02515 (KGP22_02500)	538767..538988	+	222	WP_045405097.1	DUF987 domain-containing protein	-
KGP22_RS02520 (KGP22_02505)	538998..539642	+	645	WP_045405098.1	hypothetical protein	-
KGP22_RS02525 (KGP22_02510)	539693..540052	+	360	WP_045405099.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KGP22_RS02530 (KGP22_02515)	540115..540492	+	378	WP_045405101.1	TA system toxin CbtA family protein	Toxin
KGP22_RS02535 (KGP22_02520)	540489..540980	+	492	WP_045405103.1	DUF5983 family protein	-
KGP22_RS02540 (KGP22_02525)	541010..541213	+	204	WP_023312607.1	DUF957 domain-containing protein	-
KGP22_RS02545 (KGP22_02530)	541284..542129	+	846	WP_045405106.1	DUF4942 domain-containing protein	-
KGP22_RS02550 (KGP22_02535)	542273..543070	+	798	WP_047359029.1	helix-turn-helix transcriptional regulator	-
KGP22_RS02555	543350..543496	+	147	Protein_488	integrase	-
KGP22_RS02560 (KGP22_02540)	543643..543888	-	246	WP_223582367.1	ogr/Delta-like zinc finger family protein	-
KGP22_RS02565 (KGP22_02545)	543885..544211	-	327	WP_223582370.1	hypothetical protein	-
KGP22_RS02570 (KGP22_02550)	544217..544396	+	180	Protein_491	IS3 family transposase	-
KGP22_RS02575 (KGP22_02555)	544939..545358	+	420	WP_223581097.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	512284..552607	40323

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (2-112)

Antitoxin

• Antitoxin CbeA (16-113)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14020.87 Da
• Isoelectric Point: 6.8696

>T201694 WP_045405101.1 NZ_CP074159:540115-540492 [Enterobacter sp. JBIWA005]
MQTQSIHPVRATASRPSPVDIWQTLLTYLLEQHYGLTLNDTPFGNDRVIQEHIEAGISLCDGVNFIVEKYELVRIDRHGF
STETQSPLIGSIDILRARKATGLMTRNDYKVVTDITTGKYSGGTR

Nucleotide

• Length: 378 bp

>T201694 NZ_CP098719:2073835-2074293 [Yersinia ruckeri]
GTGATGCTCTACCGGATAGTTCACAACCGTTACCTGGCAACGGCCTGGACCGGATATGGCGCAGAAACCTATGGTGGCCG
CTGGAATCATAAAGGCCATGCGGCGATTTATCTGGCCAGTAGCGTTTCTCTGGCGATGCTCGAAACACTGGTGCATGTAG
AAGATAGCGCGACGCTGAACGATTTTAATCTATTTCAGATAACCATTCCCGATAATGCAATTATGGTACTGCAACAGCAA
GATTGGCCACGTGGATGGCGAGATGACCCCACCCCCGTGGCCACCATGGACATCGGCACCGAATGGATTGGTTCAGTATC
ATCTGTCGGTCTGCTGGTACCATCCACGTTAGTCCCTTTGGAAAGAAATATGCTGGTGAATCCTAAACACCGCGATTTCG
CCTCGTGCCTGAAAAGCATCAATCCACTGACGTTCAGTTTTGATCCTCGTCTAAAGTAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13267.07 Da
• Isoelectric Point: 4.5637

>AT201694 WP_045405099.1 NZ_CP074159:539693-540052 [Enterobacter sp. JBIWA005]
MPDINTTQNDNMDAPSWGLHRDITPCFGARLVQEGNCLHYLADRASITGQFSEADLRHLDQVFPLMLKQLELMLVSGELN
PRHQHCVTLYYNGLICEADTLGSCGYIYLAIYPGEPPAA

Nucleotide

• Length: 360 bp

>AT201694 NZ_CP098719:2073375-2073821 [Yersinia ruckeri]
ATGAGAACTTATCACCCAAGCCCGATGGCCAAAGTCAGCGCACTATGGCGTGAGATTGGTCTACCCGCCAGTCGTGGCAC
CGCTTTGGTGGACAGCATAAAACTGGGATTTACTGTGGATGTGATCGACACTATACATTTATGGGCTGATATGCCTAAAT
CTGAAATATTACGCGCTACGGGCATTCCGAGCCGCAGTCTGACGCGCCGTCGTACCCATGATGGGCGTTTTACCCCGGAA
GAAAGCGAACGTATTGCACGGTTTGTCCGGGTTATGGATGCGGCGGTAGATTTGTTCGGTGGTGACCGCACCAAAGCAAC
CGCATGGATGTCGATGCCGGTCAAAGGGCTGGGTAATCGAACGCCCGATTCACTGCTGGAAACCGAAACCGGTGCGTTGG
AAATCTGCGACCTGATTGGTCGTCTCGAACATGGGGTCTTTTCGTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D2T8