Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 37887..38543 Replicon plasmid pJBIWA007_2
Accession NZ_CP074156
Organism Enterobacter asburiae strain JBIWA007

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag KGP18_RS23075 Protein ID WP_223538236.1
Coordinates 37887..38069 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag KGP18_RS23080 Protein ID WP_063161120.1
Coordinates 38112..38543 (+) Length 144 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGP18_RS23035 (KGP18_22965) 33896..34207 - 312 WP_223538213.1 DUF4406 domain-containing protein -
KGP18_RS23040 (KGP18_22970) 34207..34410 - 204 WP_223538217.1 hypothetical protein -
KGP18_RS23045 (KGP18_22975) 34447..34935 - 489 WP_245403433.1 DUF2514 domain-containing protein -
KGP18_RS23050 (KGP18_22980) 34992..35537 - 546 WP_223538222.1 lysozyme -
KGP18_RS23055 (KGP18_22985) 35509..35778 - 270 WP_223538225.1 phage holin -
KGP18_RS23060 (KGP18_22990) 35892..36971 - 1080 WP_223538228.1 site-specific DNA-methyltransferase -
KGP18_RS23065 (KGP18_22995) 37238..37465 - 228 WP_223538230.1 hypothetical protein -
KGP18_RS23070 (KGP18_23000) 37477..37674 - 198 WP_223538233.1 hypothetical protein -
KGP18_RS23075 (KGP18_23005) 37887..38069 + 183 WP_223538236.1 type II toxin-antitoxin system HicA family toxin Toxin
KGP18_RS23080 (KGP18_23010) 38112..38543 + 432 WP_063161120.1 transcriptional regulator Antitoxin
KGP18_RS23085 (KGP18_23015) 38565..38795 + 231 WP_223538239.1 hypothetical protein -
KGP18_RS23090 (KGP18_23020) 38795..40180 + 1386 WP_223538242.1 RNA-guided endonuclease TnpB family protein -
KGP18_RS23095 (KGP18_23025) 40187..40375 + 189 Protein_49 helix-turn-helix transcriptional regulator -
KGP18_RS23100 (KGP18_23030) 40731..40952 - 222 WP_223538244.1 hypothetical protein -
KGP18_RS23105 (KGP18_23035) 40971..41312 - 342 WP_223538247.1 hypothetical protein -
KGP18_RS23110 (KGP18_23040) 41474..41893 - 420 WP_223538249.1 hypothetical protein -
KGP18_RS23115 (KGP18_23045) 41897..42193 - 297 WP_223538252.1 hypothetical protein -
KGP18_RS23120 42195..42821 - 627 WP_223538255.1 hypothetical protein -
KGP18_RS23125 (KGP18_23055) 42818..43021 - 204 WP_223538257.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..47243 47243


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-82)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6809.92 Da        Isoelectric Point: 10.9041

>T201693 WP_223538236.1 NZ_CP074156:37887-38069 [Enterobacter asburiae]
VKYSEFRRWLVQQGAEFKKAPGGGSHQKVSLNGKRSVFPDHGSKEMPEPLRKKIMKDLGL

Download         Length: 183 bp

>T201693 NZ_CP098719:c1106785-1106582 [Yersinia ruckeri]
ATGACCAAAACTGACTACCTAATGCGTTTAAGAAAATGCACAACAATTGACACACTAGAGCGTGTTATTGAAAAAAATAA
GTATGAGCTATCTGATGATGAACTAGAATTATTCTATTCTGCTGCGGATCATCGTCTAGCTGAACTTACCATGAATAAGT
TATACGATAAAATTCCTGCAACTGTCTGGCAACATGTGAAGTAA

Antitoxin


Download         Length: 144 a.a.        Molecular weight: 15648.88 Da        Isoelectric Point: 4.8509

>AT201693 WP_063161120.1 NZ_CP074156:38112-38543 [Enterobacter asburiae]
MFNYPVKLERDDETGAYVVSCRDLPLFNSVGDSVEEALLEASYGLVAAVAIEIEERRPVPTGSEPKEGEYVVSLPVLPAM
KAALHNAMIETGTRKAELARKLGKNGTQIDRLLDVEHSSKVETIELALHQLNKNIGVSVTSMR

Download         Length: 432 bp

>AT201693 NZ_CP098719:c1107205-1106837 [Yersinia ruckeri]
ATGGATGAGTACTCACCTAAGCGACATGATATTGCTCAGTTGAAATTCTTATGTGAGAATTTGTATGATGAAGGTATCGC
CACATTGGGCGATAGCCACCACGGCTGGGTTAATGATCCAACCTCCGCGGTAAATCTCCAACTGAATGATTTAATCGAAC
ACATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACGCAGACGAAATCGATCTCTGCGAGTTGGTGGAGGAATATCTA
GACGATACATATACCTTGTTCAGCAGTTACGGAATCAATGATCCTGATTTACGCCGCTGGCAGAAAACAAAAGACCGATT
ATTCAGACTATTTTCAGGGGAATACCTATGTGCCCTGATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References