Detailed information of TA system TA201690

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): KacT-ataR/DUF1778(antitoxin)
• Location: 2944319..2945116
• Replicon: chromosome
• Accession: NZ_CP074154
• Organism: Enterobacter asburiae strain JBIWA007

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: KGP18_RS14290
• Protein ID: WP_223537907.1
• Coordinates: 2944319..2944819 (-)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: KGP18_RS14295
• Protein ID: WP_023311079.1
• Coordinates: 2944847..2945116 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP18_RS14260 (KGP18_14240)	2939902..2940591	+	690	WP_028012657.1	pyrimidine utilization protein B	-
KGP18_RS14265 (KGP18_14245)	2940603..2940989	+	387	WP_010429508.1	pyrimidine utilization protein C	-
KGP18_RS14270 (KGP18_14250)	2940997..2941797	+	801	WP_223534447.1	pyrimidine utilization protein D	-
KGP18_RS14275 (KGP18_14255)	2941807..2942397	+	591	WP_109844504.1	malonic semialdehyde reductase	-
KGP18_RS14280 (KGP18_14260)	2942407..2942901	+	495	WP_223537903.1	pyrimidine utilization flavin reductase protein F	-
KGP18_RS14285 (KGP18_14265)	2942923..2944245	+	1323	WP_223534449.1	pyrimidine utilization transport protein G	-
KGP18_RS14290 (KGP18_14270)	2944319..2944819	-	501	WP_223537907.1	GNAT family N-acetyltransferase	Toxin
KGP18_RS14295 (KGP18_14275)	2944847..2945116	-	270	WP_023311079.1	DUF1778 domain-containing protein	Antitoxin
KGP18_RS14300 (KGP18_14280)	2945178..2946095	-	918	WP_223534451.1	DMT family transporter	-
KGP18_RS14305 (KGP18_14285)	2946213..2946383	-	171	WP_023311077.1	general stress protein	-
KGP18_RS14310 (KGP18_14290)	2946774..2947370	+	597	WP_023311076.1	NAD(P)H:quinone oxidoreductase	-
KGP18_RS14315 (KGP18_14295)	2947391..2947618	+	228	WP_008499832.1	YccJ family protein	-
KGP18_RS14320 (KGP18_14300)	2947652..2948893	-	1242	WP_223534453.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
KGP18_RS14325 (KGP18_14305)	2949046..2949489	+	444	WP_223534454.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (2-88)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18999.69 Da
• Isoelectric Point: 7.9145

>T201690 WP_223537907.1 NZ_CP074154:c2944819-2944319 [Enterobacter asburiae]
MLSEGTDYDFEDFDCGEPSLNTFLAEHLVRQHNGRILRAYLLKERDRPRVLGYYTLSGSCFERAMLPSKTQQRRIPYINV
PSVTLGRLAVDKTLQGNEWGTTLVAHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKQGFIPLTAENSHSLFFPTKSIE
RLFEQE

Nucleotide

• Length: 501 bp

>T201690 NZ_CP098717:c68559-68236 [Yersinia ruckeri]
ATGGAGTATCTAGAATTCATTGAGACCCCCGTATTTAGCAGGCGAAGAGCTGAACTGCTGCCAGATGATGACTTCAGAGC
TTTACAGGAGCATCTTCTTAAAAACCATGATCTTGGCGACACAATCAGTAAAACAGGTGGATGTAAGAAAATCCGCTGGA
GTCGCGAAGGCATGGGGAAGCGGGGGGGAGTAAGAGTTATTTATTACGTCATAACCAAATCGGGACGCCTTTATCTGCTA
CTGGTCTATCCCAAAAACGAAAAAGACGATCTGACTGAAGCCGAAAAAGCTGTCCTGAAATCGATAAGTCAGCAAATCGA
ATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10249.72 Da
• Isoelectric Point: 5.8836

>AT201690 WP_023311079.1 NZ_CP074154:c2945116-2944847 [Enterobacter asburiae]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWTAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT201690 NZ_CP098717:c68190-67894 [Yersinia ruckeri]
ATGAAAACTGAACTGTTTAATGATCTCGTTGAAAGTATGCAACAGATGGTTGCTATTGAGAAAGGTACGCTCTCTGTTCC
AGCGGAAAACATCCATCGTCATCGACTTCCAGATGTAAAATCAATGCGTGAAGCGAGCGGTCTTAAGCAAAATGAATTTG
CGGATGTAGTGGGGGTTTCTGCTTCTCTGCTCCAGAGCTGGGAACAGCATCGTCGTATTCCATCAGGTTCATCTCTAAAG
TTGTTGATGATGATAGAGCGTGACCCAGCTGTTATAGACGCTTTACGAACGCTTTAG