Detailed information of TA system TA201676

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3966937..3967594
• Replicon: chromosome
• Accession: NZ_CP074149
• Organism: Enterobacter sp. JBIWA008

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: W7P4L3
• Locus tag: KGP24_RS19090
• Protein ID: WP_023326024.1
• Coordinates: 3966937..3967347 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W7NX65
• Locus tag: KGP24_RS19095
• Protein ID: WP_006178375.1
• Coordinates: 3967328..3967594 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP24_RS19070 (KGP24_18955)	3962930..3964663	-	1734	WP_223561480.1	single-stranded-DNA-specific exonuclease RecJ	-
KGP24_RS19075 (KGP24_18960)	3964669..3965382	-	714	WP_029740340.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
KGP24_RS19080 (KGP24_18965)	3965411..3966307	-	897	WP_023309098.1	site-specific tyrosine recombinase XerD	-
KGP24_RS19085 (KGP24_18970)	3966409..3966930	+	522	WP_223561481.1	flavodoxin FldB	-
KGP24_RS19090 (KGP24_18975)	3966937..3967347	-	411	WP_023326024.1	protein YgfX	Toxin
KGP24_RS19095 (KGP24_18980)	3967328..3967594	-	267	WP_006178375.1	FAD assembly factor SdhE	Antitoxin
KGP24_RS19100 (KGP24_18985)	3967889..3968869	+	981	WP_223561482.1	tRNA-modifying protein YgfZ	-
KGP24_RS19105 (KGP24_18990)	3968943..3969602	-	660	WP_023333307.1	hemolysin III family protein	-
KGP24_RS19110 (KGP24_18995)	3969768..3970079	-	312	WP_223561483.1	N(4)-acetylcytidine aminohydrolase	-
KGP24_RS19115 (KGP24_19000)	3970131..3970862	+	732	WP_223563527.1	MurR/RpiR family transcriptional regulator	-
KGP24_RS19120 (KGP24_19005)	3970979..3972412	+	1434	WP_223561484.1	6-phospho-beta-glucosidase BglA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16063.06 Da
• Isoelectric Point: 10.9468

>T201676 WP_023326024.1 NZ_CP074149:c3967347-3966937 [Enterobacter sp. JBIWA008]
VVLWQSDLRVSWRSQWMSLLLHGLVAAIVLLMPWPLSYTPLWLLLLSFVVFDSVRSQRRINARQGEIKLLMDSRLRWQGK
EWDILGMPWMLNCGMMLRLRKVDGGRCQHLWLAADSMDAAEWRDLRRMLLQQTTQG

Nucleotide

• Length: 411 bp

>T201676 NZ_CP098714:457650-458306 [Yersinia ruckeri]
GTGGCCTATGACTACAGCACTAGCCTCAACCCGGAAAAAGCCTTGATCTGGCGCATTGTCCATCGGGACAATATTCCGTG
GATACTGGACAACGGTCTGCACTGTGGCAACAGTCCGGTGCAGGCGGAAGACTGGATTAATATCGGTAACCCGGAGCTTA
TCGACAAACGTGCCGGACATCTGGTGCCAACCGGAATGCGGGGGACGCTGCATGACTATGTGCCGTTTTACTTTACTCCT
TTCTCGCCGATGCTGATGAATATCCGCAGCGGACGTGGCGGCATTAAGCAACGCCCCAATGAGGAGATCGTTATTCTGGT
CAGTAGCCTGCGAAACATGGTGGCGCAAAATGTCTCCTTCGTGTTTACCGATAGCCACGCATATTATAACCTGGCTAATT
ACTTTACCGAGCTGGACAGTCTGAACCAGATCGATTGGCCCATTCTACAAGCGCGGGATTTCCGTCGCGATCAGGACGAT
TTGGCCAAGTTCGAACGCTATCAGGCAGAGGCGTTAATTTGGAAGCACTGCCCAATAGCGCTGCTTGGCGGGATGATTTG
TTACAACGATGGCGTCAGGTTACAATTAGAGCAGTGGTTGTCGCAACGCAATCTGTCAATGCCCGTTCACGCGCGGGCTG
GGTGGTACTTCTCATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10569.05 Da
• Isoelectric Point: 5.1340

>AT201676 WP_006178375.1 NZ_CP074149:c3967594-3967328 [Enterobacter sp. JBIWA008]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDDDKRLFVRLLESDDPDLFNWLMNHGKPADTELQRMVQLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT201676 NZ_CP098714:458303-459370 [Yersinia ruckeri]
ATGATTACATTTACACAAGGCAATTTACTGGATGCGCCCGTAGAAGCACTAGTGAATACGGTTAATACCGTCGGTGTCAT
GGGTAAAGGCATCGCGCTGATGTTCAAAGAGCGCTTCCCCGTCAATATGAAAGCCTATGCTCTCGCCTGCAAACATAAGC
AGGTAATGACAGGTAAAATGTTTATTACCGAAACCGGCGAACTGATGGGGCCTCGCTGGATTGTCAACTTCCCAACTAAA
CAACACTGGCGCGCAGACTCCCGCATGGAGTGGGTTGAAGACGGCTTGCAGGATTTACGCCGCTTTTTGATCGAAGAGAA
AGTGCAATCTATCGCCATTCCCCCACTGGGGGCGGGTAACGGCGGCCTGAATTGGTCGGACGTTCGGATGCGGATTGAAT
CTGCGCTGGGCGACCTTCAGGACACGGAAATTCTGATTTATGAGCCTACCGAAAAATACCAGAACGTCGCCAAGAGCACC
GGCGTAAAAAAGCTCACTCCCGCCAGAGCGCTGATTGCTGAACTGGTGCGCCGCTACTGGGTGCTGGGCATGGAGTGCAG
CCTGCTGGAAATCCAGAAGCTGGCATGGCTGTTACAACGCGCCATTGAACAGCATCAGCAGGAAGATGTGCTGAAACTGC
GGTTTGAGGCGCACTATTACGGCCCCTACGCCCCCAACCTTAACCATCTGCTTAACGCGCTAGATGGCACGTATCTGAAA
GCAGAGAAACGCATCCCGGACACCCAGCCGCTGGATGTGATCTGGTTTAATGATCAGGAAAAAGAACACATCAACCTCTG
GCTGAACAACGAGGCCAAAGAGTGGCTACCGGCGCTTGAGCAGGTCAGTCAGTTGATTGACGGCTTTGAATCGCCGTTTG
GTCTGGAGCTGCTGGCAACCGTTGACTGGCTGCTGACCCGAGGAAAATGCCAGCCCACGCTGGATTCCGTGAAAGAGGGG
CTGCACCAGTGGCCTGCGGGTGAACGCTGGGCCAGCCGTAAGATGAAGCTCTTTGACGACAAGAATCTGCAATTTGCCAT
CAACCGCGTGATGGAGTTCCATTGCTAA

Structures

Toxin

• AlphaFold DB: W7P4L3

Antitoxin

• AlphaFold DB: W7NX65