Detailed information of TA system TA201665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1129599..1130219
• Replicon: chromosome
• Accession: NZ_CP074149
• Organism: Enterobacter sp. JBIWA008

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: KGP24_RS05495
• Protein ID: WP_148244022.1
• Coordinates: 1129599..1129817 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: V3PBI9
• Locus tag: KGP24_RS05500
• Protein ID: WP_008499288.1
• Coordinates: 1129845..1130219 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP24_RS05465 (KGP24_05455)	1125613..1125873	+	261	WP_010428154.1	type B 50S ribosomal protein L31	-
KGP24_RS05470 (KGP24_05460)	1125876..1126016	+	141	WP_003859006.1	type B 50S ribosomal protein L36	-
KGP24_RS05475 (KGP24_05465)	1126013..1126723	-	711	WP_223562614.1	GNAT family protein	-
KGP24_RS05480 (KGP24_05470)	1126825..1128285	+	1461	WP_223562615.1	PLP-dependent aminotransferase family protein	-
KGP24_RS05485 (KGP24_05475)	1128257..1128724	-	468	WP_223562616.1	YlaC family protein	-
KGP24_RS05490 (KGP24_05480)	1128842..1129393	-	552	WP_223562617.1	maltose O-acetyltransferase	-
KGP24_RS05495 (KGP24_05485)	1129599..1129817	-	219	WP_148244022.1	HHA domain-containing protein	Toxin
KGP24_RS05500 (KGP24_05490)	1129845..1130219	-	375	WP_008499288.1	Hha toxicity modulator TomB	Antitoxin
KGP24_RS05505 (KGP24_05495)	1130730..1133876	-	3147	WP_223562618.1	multidrug efflux RND transporter permease subunit AcrB	-
KGP24_RS05510 (KGP24_05500)	1133899..1135092	-	1194	WP_223562619.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8646.02 Da
• Isoelectric Point: 8.9008

>T201665 WP_148244022.1 NZ_CP074149:c1129817-1129599 [Enterobacter sp. JBIWA008]
MSDKPLTKFDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Nucleotide

• Length: 219 bp

>T201665 NZ_CP098710:c2889526-2889104 [Yersinia ruckeri]
GTGGCCCAGTGGCGATGTGATCTGCGGGTTTCATGGCGCACCCAACTTTTCTCACTATTGGTACACGGTGTTCTGGTATT
ACTGACACTGGTGGTACCTTGGCCTGACGGATTTACTCCGCTATGGCTTATATTGCTGACGCTGGTGGTGTTTGAATGCA
TTCGCAGCCAGAAGAACATTACCTCTCGACAGGGAGAGATCCGGCTTAAAGCGGGAAATCTGTTGATTTGGAAGTGGCAA
GAGTGGACGTTGGTGCGGCCGCCGTGGATTACCCGCTACGGCGTATTATTATCGTTACAAGGCGCTGGCAGCCGCCGCCG
AAAACGTATTTGGCTAGCCTCCGACAGCATGTCGGAAGAAAAATGGCGCGAGTTGTGTCTGATATTACGACATACTTTTG
ATTCTGCGGCAGGCCGTGAGTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14481.28 Da
• Isoelectric Point: 4.8886

>AT201665 WP_008499288.1 NZ_CP074149:c1130219-1129845 [Enterobacter sp. JBIWA008]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Nucleotide

• Length: 375 bp

>AT201665 NZ_CP098710:c2889773-2889507 [Yersinia ruckeri]
ATGGAAATTGATAACAAAGCCCGAATCCATTGGGCATGCCGCCGGGGTATGCGTGAACTGGATATCTCCATAATGCCATT
TTTCGAACATGAATATGACACGCTAAACGATAATGATAAGCGCGTATTTATCCGCTTGCTGACGAGTGATGATCCTGATT
TATTCAATTGGTTGATGAACCACGGCGAGCCGCAGGATAGCGAACTGAAACGAATGGTTAAACTGATTCAGACACGAAAT
AAAGCCCGTGGCCCAGTGGCGATGTGA

Structures

Antitoxin

• AlphaFold DB: V3PBI9