Detailed information of TA system TA201662

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 83206..84005
• Replicon: chromosome
• Accession: NZ_CP074149
• Organism: Enterobacter sp. JBIWA008

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: KGP24_RS00405
• Protein ID: WP_223561992.1
• Coordinates: 83206..83583 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: KGP24_RS00410
• Protein ID: WP_223561993.1
• Coordinates: 83640..84005 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP24_RS00385 (KGP24_00385)	80399..81592	-	1194	WP_223561988.1	IS4 family transposase	-
KGP24_RS00390 (KGP24_00390)	81671..82354	-	684	WP_223561989.1	DUF4942 domain-containing protein	-
KGP24_RS00395 (KGP24_00395)	82521..82688	-	168	WP_223561990.1	hypothetical protein	-
KGP24_RS00400 (KGP24_00400)	82718..83209	-	492	WP_223561991.1	DUF5983 family protein	-
KGP24_RS00405 (KGP24_00405)	83206..83583	-	378	WP_223561992.1	TA system toxin CbtA family protein	Toxin
KGP24_RS00410 (KGP24_00410)	83640..84005	-	366	WP_223561993.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KGP24_RS00415 (KGP24_00415)	84028..84249	-	222	WP_223561994.1	DUF987 domain-containing protein	-
KGP24_RS00420 (KGP24_00420)	84263..84742	-	480	WP_223561995.1	DNA repair protein RadC	-
KGP24_RS00425 (KGP24_00425)	84754..85236	-	483	WP_223561996.1	antirestriction protein	-
KGP24_RS00430 (KGP24_00430)	85247..85495	-	249	WP_223561997.1	hypothetical protein	-
KGP24_RS00435 (KGP24_00435)	85495..86313	-	819	WP_223561998.1	DUF932 domain-containing protein	-
KGP24_RS00440 (KGP24_00440)	86534..86890	-	357	WP_223561999.1	hypothetical protein	-
KGP24_RS00445 (KGP24_00445)	86972..87283	-	312	WP_166770358.1	hypothetical protein	-
KGP24_RS00450 (KGP24_00450)	87295..87561	-	267	WP_223562000.1	hypothetical protein	-
KGP24_RS00455	87616..88119	-	504	WP_223562001.1	hypothetical protein	-
KGP24_RS00460 (KGP24_00460)	88116..88844	-	729	WP_223562002.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13943.00 Da
• Isoelectric Point: 9.6551

>T201662 WP_223561992.1 NZ_CP074149:c83583-83206 [Enterobacter sp. JBIWA008]
MQTLSAISVRKASSRPTPVKIWQQLLTYLLERHYGLSLNDTQFGNDCVIQQHIDAGISLADALNFLVEKSELVRIDRPGF
SIQHQSPFIGVIDILRARKATGLMQRTGYKAVTCAISGQSSREQQ

Nucleotide

• Length: 378 bp

>T201662 NZ_CP098710:c915005-914802 [Yersinia ruckeri]
ATGACCAAAACTGACTACCTAATGCGTTTAAGAAAATGCACAACAATTGACACACTAGAGCGTGTTATTGAAAAAAATAA
GTATGAGCTATCTGATGATGAACTAGAATTATTCTATTCTGCTGCGGATCATCGTCTAGCTGAACTTACCATGAATAAGT
TATACGATAAAATCCCTGCAACTGTCTGGCAACATGTGAAGTAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13425.32 Da
• Isoelectric Point: 6.6238

>AT201662 WP_223561993.1 NZ_CP074149:c84005-83640 [Enterobacter sp. JBIWA008]
MQNTTNVANHDIAEPWWGLKPTVTPCFGARLVQEGNRLHYLADRASIIGQFSDADLRHLDQAFPVLLKQLELMLVSGELN
PRHQHCVTLYGKGLTCDADTLGSCGYLYIAIYPTSANSIPR

Nucleotide

• Length: 366 bp

>AT201662 NZ_CP098710:c915425-915057 [Yersinia ruckeri]
ATGGATGAGTACTCACCTAAGCGACATGATATTGCTCAGTTGAAATTCTTATGTGAGAATTTGTATGATGAAGGTATCGC
CACATTGGGCGATAGCCACCACGGCTGGGTTAATGATCCAACCTCCGCGGTAAATCTCCAACTGAATGATTTAATCGAAC
ACATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACGCAGACGAAATCGATCTCTGCGAGTTGGTGGAGGAATATCTA
GACGATACATATACCTTGTTCAGCAGTTACGGAATCAATGATCCTGATTTACGCCGCTGGCAGAAAACAAAAGACCGATT
ATTCAGACTATTTTCAGGGGAATACCTATGTGCCCTGATGAAAAATTAA