Detailed information of TA system TA201632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 50925..51444
• Replicon: plasmid pJSRIV002_1
• Accession: NZ_CP074144
• Organism: Serratia sp. JSRIV002

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KGP26_RS28780
• Protein ID: WP_223499778.1
• Coordinates: 50925..51212 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KGP26_RS28785
• Protein ID: WP_223499777.1
• Coordinates: 51202..51444 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP26_RS28755	46026..46538	-	513	WP_223499781.1	hypothetical protein	-
KGP26_RS28760 (KGP26_28680)	47085..47252	-	168	Protein_58	transposase	-
KGP26_RS28765 (KGP26_28685)	47403..48263	+	861	WP_223499780.1	class I SAM-dependent methyltransferase	-
KGP26_RS28770 (KGP26_28690)	48330..49025	-	696	WP_223499779.1	IS6 family transposase	-
KGP26_RS28775 (KGP26_28695)	49875..50675	+	801	WP_223499828.1	tyrosine-type recombinase/integrase	-
KGP26_RS28780 (KGP26_28700)	50925..51212	-	288	WP_223499778.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KGP26_RS28785 (KGP26_28705)	51202..51444	-	243	WP_223499777.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
KGP26_RS28790 (KGP26_28710)	51747..52940	+	1194	WP_223499776.1	AAA family ATPase	-
KGP26_RS28795 (KGP26_28715)	52949..53920	+	972	WP_223499775.1	ParB family protein	-
KGP26_RS28800 (KGP26_28720)	54493..55227	+	735	WP_223499774.1	N-6 DNA methylase	-
KGP26_RS28805 (KGP26_28725)	55152..55757	+	606	WP_223499773.1	IS66-like element accessory protein TnpA	-
KGP26_RS28810 (KGP26_28730)	55754..56101	+	348	WP_223499772.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	fimD / fimC / pilW	1..117877	117877

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10979.92 Da
• Isoelectric Point: 10.3023

>T201632 WP_223499778.1 NZ_CP074144:c51212-50925 [Serratia sp. JSRIV002]
MTYKIDFDNAAFKEWKKLGETVRQQLKKKLAQIVEAPRVEANRLHSLPDCYKIKLRASGYRLVYKVEDETVVILVLAVGK
RERLEVYKAAATRLG

Nucleotide

• Length: 288 bp

>T201632 NZ_CP098698:22916-23206 [Yersinia ruckeri]
ATGATTAAGTCTTTTCGGGATAAAAACCTAGAACTGTTCTACATGGAAAGTAAACGAGATCGCGCGATACCGGCAACAAT
AGAGCGCCAGTTAGCGAAGAAGCTTGATATGTTGGCCGCCGCACATAATGAGCGAGATTTGTTTATTCCAACCAGTAATT
ACTACAAGCGTCTCTCAGGGCAGTTTGATGGCTGGTCAAGTATGAGAGTCAATATTCAATGGCGGTTAATGTTTCAATGG
AAAGACGGATATGCTGAAAGCATCTACCTCGATCCTCATCAAGACACCTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8726.03 Da
• Isoelectric Point: 4.3951

>AT201632 WP_223499777.1 NZ_CP074144:c51444-51202 [Serratia sp. JSRIV002]
MGTMNIRIDDDLKARAGVALDGLGITASEAVRLVLEYVAQNNRLPVQVALLADEDADIIELARERLANPGKRIRVTLDDL

Nucleotide

• Length: 243 bp

>AT201632 NZ_CP098698:23218-23547 [Yersinia ruckeri]
ATGCTTGATACCACTACTCGCGAACCTACTACGGTAGGCGAAATTTTGCAGGAAGAATTTTTAACCCCATTAGCCATTGG
GCAGGAAGAACTGGCCGATATTATGGGCGTGACTCGCGCAACGGTTAACCGTTTGTGCAAAGGCCGTCGTCGTCTGACTG
TAGAGGAAGCGACGCTGTTTTCTGAGTTGTTTGAGACGACGCCCGAATTTTGGTTGAACCTCCAAGCCGCTCATGACCGT
TGGGAAGCACGAGAAGCGCTTCAAAAACATAAGCGACCCCCAATGCGTCCTATCATGGAAATGTTGGGACGGCGTATCTC
ACACGCCTGA