Detailed information of TA system TA201631

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 12175..12752
• Replicon: plasmid pJSRIV002_1
• Accession: NZ_CP074144
• Organism: Serratia sp. JSRIV002

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KGP26_RS28535
• Protein ID: WP_223498409.1
• Coordinates: 12175..12507 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: KGP26_RS28540
• Protein ID: WP_223498408.1
• Coordinates: 12507..12752 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP26_RS28510 (KGP26_28425)	7776..8171	-	396	WP_223498390.1	hypothetical protein	-
KGP26_RS28515 (KGP26_28430)	8702..9543	-	842	Protein_9	ISKra4 family transposase	-
KGP26_RS28520 (KGP26_28435)	9723..10268	-	546	WP_245398329.1	plasmid pRiA4b ORF-3 family protein	-
KGP26_RS28525 (KGP26_28440)	11153..11323	-	171	WP_223498411.1	hypothetical protein	-
KGP26_RS28530 (KGP26_28445)	11847..12161	-	315	WP_223498410.1	hypothetical protein	-
KGP26_RS28535 (KGP26_28450)	12175..12507	-	333	WP_223498409.1	endoribonuclease MazF	Toxin
KGP26_RS28540 (KGP26_28455)	12507..12752	-	246	WP_223498408.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KGP26_RS28545 (KGP26_28460)	12920..13126	-	207	WP_223498407.1	hypothetical protein	-
KGP26_RS28550 (KGP26_28465)	13284..13514	-	231	WP_223498406.1	hypothetical protein	-
KGP26_RS28555 (KGP26_28470)	13511..13834	-	324	WP_223498405.1	hypothetical protein	-
KGP26_RS28560 (KGP26_28475)	13857..14096	-	240	WP_223498404.1	helix-turn-helix domain-containing protein	-
KGP26_RS28565 (KGP26_28480)	14358..14852	-	495	WP_223498403.1	hypothetical protein	-
KGP26_RS28570 (KGP26_28485)	15081..15908	-	828	WP_223498402.1	phosphoenolpyruvate hydrolase family protein	-
KGP26_RS28575 (KGP26_28490)	15905..16366	-	462	WP_223538574.1	Tm-1-like ATP-binding domain-containing protein	-
KGP26_RS28580 (KGP26_28495)	16444..17139	-	696	WP_223498400.1	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	fimD / fimC / pilW	1..117877	117877
	-	flank	IS/Tn	-	-	8713..9543	830

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-107)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11901.85 Da
• Isoelectric Point: 8.4953

>T201631 WP_223498409.1 NZ_CP074144:c12507-12175 [Serratia sp. JSRIV002]
MVKGYVPDAGDIVWLEFDPQAGHEQAGHRPALVLSPAVYNGRIGLMLCCPMTTKIKGYPFEVIVPGERESAVLADQVKSL
DWKERNATKKGQVPASVLSDVRAKAKALIG

Nucleotide

• Length: 333 bp

>T201631 NZ_CP098697:c3007636-3007214 [Yersinia ruckeri]
GTGGCCCAGTGGCGATGTGATCTGCGGGTTTCATGGCGCACCCAACTTTTCTCACTATTGGTACACGGTGTTCTGGTATT
ACTGACACTGGTGGTACCTTGGCCTGACGGATTTACTCCGCTATGGCTTATATTGCTGACGCTGGTGGTGTTTGAATGCA
TTCGCAGCCAGAAGAACATTACCTCTCGACAGGGAGAGATCCGGCTTAAAGCGGGAAATCTGTTGATTTGGAAGTGGCAA
GAGTGGACGTTGGTGCGGCCGCCGTGGATTACCCGCTACGGCGTATTATTATCGTTACAAGGCGCTGGCAGCCGCCGCCG
AAAACGTATTTGGCTAGCCTCCGACAGCATGTCGGAAGAAAAATGGCGCGAGTTGTGTCTGATATTACGACATACTTTTG
ATTCTGCGGCAGGCCGTGAGTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8600.94 Da
• Isoelectric Point: 4.9861

>AT201631 WP_223498408.1 NZ_CP074144:c12752-12507 [Serratia sp. JSRIV002]
MSQVVVKKWGNSPSVRLPVAVMKAASLNVDDTVEVSVEEGRIVIVPVRAKVYSLDGLLAGVTDENIHQAVSFGEPVGKEA
L

Nucleotide

• Length: 246 bp

>AT201631 NZ_CP098697:c3007883-3007617 [Yersinia ruckeri]
ATGGAAATTGATAACAAAGCCCGAATCCATTGGGCATGCCGCCGGGGTATGCGTGAACTGGATATCTCCATAATGCCATT
TTTCGAACATGAATATGACACGCTAAACGATAATGATAAGCGCGTATTTATCCGCTTGCTGACGAGTGATGATCCTGATT
TATTCAATTGGTTGATGAACCACGGCGAGCCGCAGGATAGCGAACTGAAACGAATGGTTAAACTGATTCAGACACGAAAT
AAAGCCCGTGGCCCAGTGGCGATGTGA