Detailed information of TA system TA201603

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yfjZ/CbtA-CbeA
• Location: 168285..169068
• Replicon: chromosome
• Accession: NZ_CP074143
• Organism: Serratia sp. JSRIV002

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: KGP26_RS00720
• Protein ID: WP_223536916.1
• Coordinates: 168285..168617 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: KGP26_RS00725
• Protein ID: WP_223536919.1
• Coordinates: 168694..169068 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP26_RS00680 (KGP26_00675)	163319..163927	-	609	WP_223536898.1	hypothetical protein	-
KGP26_RS00685 (KGP26_00680)	164363..165250	+	888	WP_223536901.1	50S ribosome-binding GTPase	-
KGP26_RS00690 (KGP26_00685)	165313..165783	+	471	WP_223536903.1	hypothetical protein	-
KGP26_RS00695 (KGP26_00690)	165915..166271	+	357	WP_223536905.1	hypothetical protein	-
KGP26_RS00700 (KGP26_00695)	166461..166898	+	438	WP_223536907.1	antirestriction protein	-
KGP26_RS00705 (KGP26_00700)	166917..167405	+	489	WP_223536909.1	DNA repair protein RadC	-
KGP26_RS00710 (KGP26_00705)	167399..167731	+	333	WP_223536911.1	DUF5983 family protein	-
KGP26_RS00715 (KGP26_00710)	167794..168279	+	486	WP_223536913.1	hypothetical protein	-
KGP26_RS00720 (KGP26_00715)	168285..168617	+	333	WP_223536916.1	TA system toxin CbtA family protein	Toxin
KGP26_RS00725 (KGP26_00720)	168694..169068	+	375	WP_223536919.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KGP26_RS00730 (KGP26_00725)	169156..170013	+	858	WP_223536922.1	DUF4942 domain-containing protein	-
KGP26_RS00735 (KGP26_00730)	170499..171617	+	1119	WP_223536926.1	PIN domain-containing protein	-
KGP26_RS00740 (KGP26_00735)	171733..172893	-	1161	WP_223536928.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	123919..198888	74969
	-	flank	IS/Tn	-	-	171733..172893	1160

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (18-104)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12396.40 Da
• Isoelectric Point: 7.2753

>T201603 WP_223536916.1 NZ_CP074143:168285-168617 [Serratia sp. JSRIV002]
MKAIDKVLHTAPKKPALIEYQQLLAWLLDHCYGLTLNDTPYHDPLAIAQQIEYGVTVRETINELVQKFDLVRVDRPGFSL
LAQDPTLSGSDMLRVRSALGLRTPINSRLV

Nucleotide

• Length: 333 bp

>T201603 NZ_CP098691:c954312-954109 [Yersinia ruckeri]
ATGACCAAAACTGACTACCTAATGCGTTTAAGAAAATGCACAACAATTGACACACTAGAGCGTGTTATTGAAAAAAATAA
GTATGAGCTATCTGATGATGAACTAGAATTATTCTATTCTGCTGCGGATCATCGTCTAGCTGAACTTACCATGAATAAGT
TATACGATAAAATTCCTGCAACTGTCTGGCAACATGTGAAGTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13432.41 Da
• Isoelectric Point: 6.0609

>AT201603 WP_223536919.1 NZ_CP074143:168694-169068 [Serratia sp. JSRIV002]
MVKIPDPKAPNNIPDPTPFWGLQPDITPRLGARLVQQGAHLHFLSDRAGCVGAFSPEAIQALELAFPVLVETLETLLRSG
ELGPRRQHCVAVQHAGFTCEADTLGSFGYVYIAVYQTARPPVAE

Nucleotide

• Length: 375 bp

>AT201603 NZ_CP098691:c954732-954364 [Yersinia ruckeri]
ATGGATGAGTACTCACCTAAGCGACATGATATTGCTCAGTTGAAATTCTTATGTGAGAATTTGTATGATGAAGGTATCGC
CACATTGGGCGATAGCCACCACGGCTGGGTTAATGATCCAACCTCCGCGGTAAATCTCCAACTGAATGATTTAATCGAAC
ACATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACGCAGACGAAATCGATCTCTGCGAGTTGGTGGAGGAATATCTA
GACGATACATATACCTTGTTCAGCAGTTACGGAATCAATGATCCTGATTTACGCCGCTGGCAGAAAACAAAAGACCGATT
ATTCAGACTATTTTCAGGGGAATACCTATGTGCCCTGATGAAAAATTAA