Detailed information of TA system TA201594

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4533854..4534666
• Replicon: chromosome
• Accession: NZ_CP074140
• Organism: Serratia sp. JSRIV004

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: KGP21_RS21650
• Protein ID: WP_223536261.1
• Coordinates: 4534283..4534666 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: KGP21_RS21645
• Protein ID: WP_223536259.1
• Coordinates: 4533854..4534222 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP21_RS21605 (KGP21_21525)	4528968..4529843	+	876	WP_223536249.1	GTPase family protein	-
KGP21_RS21610 (KGP21_21530)	4530057..4530758	+	702	WP_042526032.1	WYL domain-containing protein	-
KGP21_RS21615 (KGP21_21535)	4530764..4531411	+	648	WP_223536251.1	hypothetical protein	-
KGP21_RS21620 (KGP21_21540)	4531537..4531680	+	144	Protein_4235	DUF4339 domain-containing protein	-
KGP21_RS21625 (KGP21_21545)	4531782..4532249	+	468	WP_223536253.1	hypothetical protein	-
KGP21_RS21630 (KGP21_21550)	4532485..4532931	+	447	WP_223536255.1	antirestriction protein	-
KGP21_RS21635 (KGP21_21555)	4532951..4533430	+	480	WP_115115199.1	DNA repair protein RadC	-
KGP21_RS21640 (KGP21_21560)	4533441..4533761	+	321	WP_223536257.1	DUF5983 family protein	-
KGP21_RS21645 (KGP21_21565)	4533854..4534222	+	369	WP_223536259.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KGP21_RS21650 (KGP21_21570)	4534283..4534666	+	384	WP_223536261.1	TA system toxin CbtA family protein	Toxin
KGP21_RS21655 (KGP21_21575)	4534663..4534884	+	222	WP_050311462.1	DUF5983 family protein	-
KGP21_RS21660 (KGP21_21580)	4534979..4535830	+	852	WP_223536264.1	DUF4942 domain-containing protein	-
KGP21_RS21665 (KGP21_21585)	4536412..4537413	+	1002	WP_223536267.1	ABC transporter substrate-binding protein	-
KGP21_RS21670 (KGP21_21590)	4537431..4538447	+	1017	WP_223498240.1	iron chelate uptake ABC transporter family permease subunit	-
KGP21_RS21675 (KGP21_21595)	4538447..4539208	+	762	WP_115160049.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14245.42 Da
• Isoelectric Point: 9.9985

>T201594 WP_223536261.1 NZ_CP074140:4534283-4534666 [Serratia sp. JSRIV004]
MQTVLVTSKRAAPSCPSPIAIWQVLMTHLLQQHYGLTLEDTAFSHEGVIQEHLNAGISLADALNFIVEKYELVRIDRTGF
SIREQSPFITPIDILRARQATGLMKRRGYKAITHITNGKNQRQGAHP

Nucleotide

• Length: 384 bp

>T201594 NZ_CP098611:c1095323-1095072 [Phormidium yuhuli AB48]
ATGGATGCCCTCAGCTACACCGCCGCTAGAAGGAATCTAGCCAAAACAATGCAGCAAGTCTGTGAGGATCACTCCCCCGT
CATCATCACCCGAAATAAAGCGGCTTCGGTGGTGATGATGTCCCTAGAGGATTACGAAGCCTTGCAAGAAACCGCCTACC
TGTTGCGTAGTCCAGCCAACGCCCGCCGTCTCTTAGAGTCTATTGCAGAACTTGAACAAGGTGGCGGCCAGGAACGAGAA
CTTTTGGAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13402.21 Da
• Isoelectric Point: 5.9492

>AT201594 WP_223536259.1 NZ_CP074140:4533854-4534222 [Serratia sp. JSRIV004]
MTDSKPDTPSQGADITPPAWGLKRIITPQVGARLVQQGHQLHYLADRADIVGKFSDAELRHLDQVFPLWLQQLESMLMSA
ELNPRQQHCVTLHASGLTLEADTLGSCGYVYISIYPTSATTA

Nucleotide

• Length: 369 bp

>AT201594 NZ_CP098611:c1095075-1094821 [Phormidium yuhuli AB48]
ATGAAGACGACGTTTTCTGAGTCAGCCTGGGATGATTACCTCTACTGGCAAAAAATGGACAAAAAAATCCTGCGTCGGGT
GAACCAACTGATTAAAGAGATTCATCGCACGCCTTTCGAGGGGATTGGTAAACCTGAGCCACTCAAACATAATCTTGCCG
GATATTGGTCTCGACGAATCACCGCTGAACAGCGAATGGTGTACAAAGTCACCCCAGACGAGCTTATGATTGCTCAGCTT
CGCTATCACTACTGA