Detailed information of TA system TA201573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 56509..57086
• Replicon: plasmid pJSRIV006_1
• Accession: NZ_CP074138
• Organism: Serratia sp. JSRIV006

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KGP16_RS26920
• Protein ID: WP_223501592.1
• Coordinates: 56509..56841 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: KGP16_RS26925
• Protein ID: WP_223501593.1
• Coordinates: 56841..57086 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP16_RS26885 (KGP16_26805)	51510..52079	-	570	WP_223498391.1	fimbrial protein	-
KGP16_RS26890 (KGP16_26810)	52582..52977	-	396	WP_223501712.1	hypothetical protein	-
KGP16_RS26895 (KGP16_26815)	53507..54348	-	842	Protein_67	ISKra4 family transposase	-
KGP16_RS26900 (KGP16_26820)	54528..55073	-	546	WP_245398329.1	plasmid pRiA4b ORF-3 family protein	-
KGP16_RS26905 (KGP16_26825)	55428..55697	-	270	WP_223501714.1	hypothetical protein	-
KGP16_RS26910 (KGP16_26830)	55810..56133	-	324	WP_223501716.1	hypothetical protein	-
KGP16_RS26915 (KGP16_26835)	56184..56495	-	312	WP_223501591.1	hypothetical protein	-
KGP16_RS26920 (KGP16_26840)	56509..56841	-	333	WP_223501592.1	endoribonuclease MazF	Toxin
KGP16_RS26925 (KGP16_26845)	56841..57086	-	246	WP_223501593.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KGP16_RS26930 (KGP16_26850)	57577..58716	+	1140	WP_223501594.1	colicin-like pore-forming protein	-
KGP16_RS26935 (KGP16_26855)	58727..59071	-	345	WP_223501596.1	colicin E1 immunity protein	-
KGP16_RS26940 (KGP16_26860)	59877..60146	-	270	WP_223501598.1	hypothetical protein	-
KGP16_RS26945 (KGP16_26865)	60425..60661	-	237	WP_223501600.1	hypothetical protein	-
KGP16_RS26950 (KGP16_26870)	60734..61591	-	858	WP_223501602.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	fimD / fimC	1..112026	112026
	-	flank	IS/Tn	-	-	53518..54348	830

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-109)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11913.94 Da
• Isoelectric Point: 9.0783

>T201573 WP_223501592.1 NZ_CP074138:c56841-56509 [Serratia sp. JSRIV006]
MVKGYVPDAGDIVWLEFDPQAGHEQAGHRPALVLSPAVYNGRIGLMLCCPMTTKIKGYPFEVIVPGERESAVLADQVKSL
DWKERNAIKKGKVPASVLSDVRAKAKALIG

Nucleotide

• Length: 333 bp

>T201573 NZ_CP098546:c1662606-1662424 [Neisseria gonorrhoeae]
TTGAATAGCCTAGACGTTATTGCCCTACTCAAACAAGACGGTTGGTATAAAGTTGCACAATCCGGGAGCCATTCGCAATA
TAAGCACCCAACAAAAAAAGGCCGTGTAACCGTGCCGCACCCGAAAAAAGACTTGCCGACAGGTACTGTAAAAAATATCT
ATAAGCAAGCCGGTTTGAAGTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8600.89 Da
• Isoelectric Point: 4.6472

>AT201573 WP_223501593.1 NZ_CP074138:c57086-56841 [Serratia sp. JSRIV006]
MSQVVVKKWGNSPSVRLPVAIMKAASLNVDDTVEVSVEEGRIVIVPVRAKVYSLDGLLAGVTDENIHQAVSFGEPVGNEA
L

Nucleotide

• Length: 246 bp

>AT201573 NZ_CP098546:c1662322-1661921 [Neisseria gonorrhoeae]
ATGTTTATCCCTGCCGCCCTGCACAAAGACGAACATTCGGCATATGGCGTAACCATCCCCGACCTTCCCGGCTGTTTCTC
TTGCGGCGACACCGTTGAAGAAGCCGTCGCCAACGCCCGTTCTGCCGCCTATATGCATATTGACGGTATGATTGAGGACG
GAGGGTTCAAAAACCTTGCCGTAAGCAGCATTGCCGATTTGAGCCAAGAACCTGATTATCATGGGGCAACATGGGTAATG
ATTGAAATCGACCCTGCAAAAATCAGTCGGCAGCAAATCCGATTCAACGTCAGCTGGCCGCAATACCTTCTTGATAGAGT
GGATGAATATACATCCGCAAATCATGAAACCCGTAGCGGTTTTTTAGCCAAAGCCGCCCTGCTCACCATGAATCAGGCAT
AG