Detailed information of TA system TA201554

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1389188..1389810
• Replicon: chromosome
• Accession: NZ_CP074137
• Organism: Serratia sp. JSRIV006

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: U2M374
• Locus tag: KGP16_RS06265
• Protein ID: WP_021180142.1
• Coordinates: 1389188..1389391 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: U2NAN4
• Locus tag: KGP16_RS06270
• Protein ID: WP_021180141.1
• Coordinates: 1389442..1389810 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP16_RS06240 (KGP16_06230)	1385015..1385503	+	489	WP_223500978.1	YbaY family lipoprotein	-
KGP16_RS06245 (KGP16_06235)	1385558..1385872	-	315	WP_021180150.1	MGMT family protein	-
KGP16_RS06255 (KGP16_06245)	1386255..1388042	-	1788	WP_223500979.1	adenine deaminase C-terminal domain-containing protein	-
KGP16_RS06260 (KGP16_06250)	1388180..1389106	+	927	WP_059200026.1	LysR family transcriptional regulator	-
KGP16_RS06265 (KGP16_06255)	1389188..1389391	-	204	WP_021180142.1	HHA domain-containing protein	Toxin
KGP16_RS06270 (KGP16_06260)	1389442..1389810	-	369	WP_021180141.1	Hha toxicity modulator TomB	Antitoxin
KGP16_RS06275 (KGP16_06265)	1389967..1390320	-	354	WP_094981218.1	hypothetical protein	-
KGP16_RS06280 (KGP16_06270)	1390700..1391392	+	693	WP_094981217.1	ABC transporter ATP-binding protein	-
KGP16_RS06285 (KGP16_06275)	1391392..1392249	+	858	WP_094981189.1	metal ABC transporter permease	-
KGP16_RS06290 (KGP16_06280)	1392275..1393153	+	879	WP_059200029.1	zinc ABC transporter substrate-binding protein	-
KGP16_RS06295 (KGP16_06285)	1393261..1393401	-	141	WP_021805462.1	type B 50S ribosomal protein L36	-
KGP16_RS06300 (KGP16_06290)	1393414..1393671	-	258	WP_021180135.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8099.48 Da
• Isoelectric Point: 6.9770

>T201554 WP_021180142.1 NZ_CP074137:c1389391-1389188 [Serratia sp. JSRIV006]
MTKIDYLMRLRKCTTIETLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPVAVWKFVR

Nucleotide

• Length: 204 bp

>T201554 NZ_CP098522:144370-144690 [Acinetobacter baumannii]
ATGTTATTCATTGAAACCAGTATTTTTACCAAGCAAATCAAAGAGCTTGTTACTGATGAAGAATATCGTCAACTCCAGCA
AGATCTATTGATACAACCTGATAAAGGAGACTTGATCAAAAATGGTGGTGGCATTCGTAAAGTCCGTTGTGCTCAGGGTA
ATAAAGGCAAAAGTGGCGGTATTCGTGTGATCTATTACTGGGTTACAGAGGATGATCAGATTTTTTTCCTAGTGGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGACAAAGAGACCTCGATCCTGCGTCAATTAGTGAAGGAGCAATTTAATGGATA
A

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14097.78 Da
• Isoelectric Point: 4.5140

>AT201554 WP_021180141.1 NZ_CP074137:c1389810-1389442 [Serratia sp. JSRIV006]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSAVNLQLNELIEHIASFVMSFKIKYMDEGDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARTFRLFSGEGVCTTMKT

Nucleotide

• Length: 369 bp

>AT201554 NZ_CP098522:144683-144955 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACCTCGTTACTTCCATCAAAGAAGTTGGAGCCATCAAACGTAATGAGATAAAGGCAAG
TCGAGTAACAGAACTTGAATTGCCTGATATTAAAGAAGTACGTGAGAAAACAGGCTTAACCCAGGCAGAGTTTGCTGCAC
GCTTACATATTAGCGCGAGAACTCTACAAAATTGGGAACAAGGTCGTCGCTATCCGACTGGCCCAGCAGCGACTTTGATT
CGTATTCTTGATACCAATCCAACTCTGATTTAG

Structures

Toxin

• AlphaFold DB: U2M374

Antitoxin

• AlphaFold DB: U2NAN4