Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 14053..14696 Replicon plasmid pEC16-CTX-55
Accession NZ_CP074124
Organism Escherichia coli strain EC16

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag KGQ53_RS25105 Protein ID WP_001044768.1
Coordinates 14280..14696 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag KGQ53_RS25100 Protein ID WP_001261287.1
Coordinates 14053..14283 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGQ53_RS25075 (10216) 10216..10596 + 381 WP_029364370.1 transposase -
KGQ53_RS25080 (10593) 10593..10940 + 348 WP_000612591.1 IS66 family insertion sequence element accessory protein TnpB -
KGQ53_RS25085 (10990) 10990..12528 + 1539 WP_072643824.1 IS66 family transposase -
KGQ53_RS25090 (12578) 12578..13477 - 900 WP_000963206.1 nucleotide-binding protein -
KGQ53_RS25095 (13467) 13467..13757 - 291 WP_000111771.1 hypothetical protein -
KGQ53_RS25100 (14053) 14053..14283 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KGQ53_RS25105 (14280) 14280..14696 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
KGQ53_RS25110 (14858) 14858..16996 - 2139 WP_000350638.1 AAA family ATPase -
KGQ53_RS25115 (17350) 17350..17607 + 258 WP_000343085.1 hypothetical protein -
KGQ53_RS25120 (17607) 17607..18197 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qnrS1 / fosA3 / blaTEM-1B / blaCTX-M-55 - 1..99826 99826


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T201539 WP_001044768.1 NZ_CP074124:14280-14696 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T201539 NZ_CP098500:c1831474-1831106 [Serratia symbiotica]
GTGGCCCAGTGGCGATGTGATGTCCGCATTTCCAGGTGTACCCAATTATTCTCGTTGCTGACGCATGGCGTCCTGGTACT
GCTGATACTGCTGCTGTTTCAAGGTATCCGCAGCCAGAAACGCCTCGCCGCAGTGCGGGGCGAGTTGCGCCTGCTGGCGG
ACTGGCGCATCAACTGGCATGGCCGCGAGTGGCGACTGGTGAAAAAACCTTGGATGCCGGATTACGGTATATTGCTGACG
CTGCACCCCATGGAGGGTAACAAGTACCAGCGGCTGTGGCTGGTTTTGGACAACATGGAGAGGGAAGAATGGCGCTATCT
GCGCCAACGTCTGCTGTATCCACCAGTTAGCGATGATGAAGAAACCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT201539 WP_001261287.1 NZ_CP074124:14053-14283 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT201539 NZ_CP098500:c1831721-1831455 [Serratia symbiotica]
ATGGATATTAACAACAAAGCACGTATCCATTGGGCCTGCCGCCGTGGCATGCGTGAGCTAGACATCTCCATCATGCCATT
CTTCGAATATGAGTATGACCAACTAAATGATACTGACAAGGCACTGTTTATCCGCCTGCTAGAGTGCGATGATCCCGATC
TGTTTAACTTGCTGATGAACCATGGCGCACCTGAGGACAGCGAACTGCAAAGAATGGTGGCCCTTATCCAGTCGCGAAAT
AAAGCCCGTGGCCCAGTGGCGATGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References