Detailed information of TA system TA201534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 1986..2533
• Replicon: plasmid pEC16-3
• Accession: NZ_CP074123
• Organism: Escherichia coli strain EC16

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G7QEJ9
• Locus tag: KGQ53_RS24420
• Protein ID: WP_001384452.1
• Coordinates: 2255..2533 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: KGQ53_RS24415
• Protein ID: WP_032181987.1
• Coordinates: 1986..2255 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGQ53_RS24410 (46)	46..1077	+	1032	WP_044706701.1	plasmid replication initiator RepA	-
KGQ53_RS24415 (1986)	1986..2255	+	270	WP_032181987.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
KGQ53_RS24420 (2255)	2255..2533	+	279	WP_001384452.1	type II toxin-antitoxin system toxin YacB	Toxin
KGQ53_RS24425 (2573)	2573..3421	+	849	WP_001057996.1	3'-5' exonuclease	-
KGQ53_RS24430 (3538)	3538..4011	+	474	WP_077128838.1	hypothetical protein	-
KGQ53_RS24435 (4488)	4488..4754	-	267	WP_000627831.1	hypothetical protein	-
KGQ53_RS24440 (4754)	4754..5248	-	495	WP_001350009.1	hypothetical protein	-
KGQ53_RS24445 (5304)	5304..5906	-	603	WP_045133169.1	ProQ/FINO family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	afaC-I	1..110400	110400

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10762.38 Da
• Isoelectric Point: 8.4615

>T201534 WP_001384452.1 NZ_CP074123:2255-2533 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T201534 NZ_CP098491:c1355858-1355112 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTACCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAACTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCCTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCCTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10177.47 Da
• Isoelectric Point: 6.7251

>AT201534 WP_032181987.1 NZ_CP074123:1986-2255 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVEQQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSATAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT201534 NZ_CP098491:c1355112-1354942 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A829CL36