Detailed information of TA system TA201533

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 33644..34442
• Replicon: plasmid pEC16-2
• Accession: NZ_CP074122
• Organism: Escherichia coli strain EC16

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: KGQ53_RS24015
• Protein ID: WP_052988649.1
• Coordinates: 33921..34442 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A141BRA2
• Locus tag: KGQ53_RS24010
• Protein ID: WP_001711191.1
• Coordinates: 33644..33913 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGQ53_RS23985	29069..30409	-	1341	WP_000137333.1	DnaB-like helicase C-terminal domain-containing protein	-
KGQ53_RS23990	30453..31193	-	741	WP_072770589.1	hypothetical protein	-
KGQ53_RS23995	31477..32244	+	768	WP_000342417.1	hypothetical protein	-
KGQ53_RS24000	32297..32650	-	354	WP_160378290.1	hypothetical protein	-
KGQ53_RS24005	32656..33324	-	669	WP_000161228.1	division plane positioning ATPase MipZ	-
KGQ53_RS24010	33644..33913	+	270	WP_001711191.1	DUF1778 domain-containing protein	Antitoxin
KGQ53_RS24015	33921..34442	+	522	WP_052988649.1	GNAT family N-acetyltransferase	Toxin
KGQ53_RS24020	34611..34862	-	252	WP_000901559.1	hypothetical protein	-
KGQ53_RS24025	34864..35556	-	693	WP_052988648.1	hypothetical protein	-
KGQ53_RS24030	35570..35893	-	324	WP_000064175.1	hypothetical protein	-
KGQ53_RS24035	35968..36750	-	783	WP_212639654.1	receptor-recognizing protein	-
KGQ53_RS25990	36817..37218	-	402	WP_171729182.1	tail fiber domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..111789	111789

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (48-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19349.10 Da
• Isoelectric Point: 7.7881

>T201533 WP_052988649.1 NZ_CP074122:33921-34442 [Escherichia coli]
VSNTTIEIFSGENDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKETLPSKSQQ
KKVPYRNVPSITLGRLALDKSLQGQGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKSLGFIQLVGNNERSL
FYSTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T201533 NZ_CP098491:504121-504471 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
CTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10054.53 Da
• Isoelectric Point: 8.7353

>AT201533 WP_001711191.1 NZ_CP074122:33644-33913 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Nucleotide

• Length: 270 bp

>AT201533 NZ_CP098491:503835-504116 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCATATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Antitoxin

• AlphaFold DB: A0A141BRA2