Detailed information of TA system TA201529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 88050..88675
• Replicon: plasmid pEC16-NDM-5
• Accession: NZ_CP074121
• Organism: Escherichia coli strain EC16

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KGQ53_RS23500
• Protein ID: WP_000911313.1
• Coordinates: 88277..88675 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: KGQ53_RS23495
• Protein ID: WP_000450520.1
• Coordinates: 88050..88277 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGQ53_RS23495 (88050)	88050..88277	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KGQ53_RS23500 (88277)	88277..88675	+	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
KGQ53_RS23505 (88684)	88684..90837	-	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
KGQ53_RS23510 (91090)	91090..91821	-	732	WP_023565183.1	conjugal transfer complement resistance protein TraT	-
KGQ53_RS23515 (91853)	91853..92350	-	498	WP_000605857.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / aac(3)-IVa / aph(4)-Ia / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..145550	145550

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T201529 WP_000911313.1 NZ_CP074121:88277-88675 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T201529 NZ_CP098486:3487698-3488078 [Enterobacter hormaechei subsp. hormaechei]
ATGCAAACTTTACCGTTACCCGAACCTCGGGCGGCTGAGCCATGTCCGTCGCCCGTGCAGGTCTGGCAACTCCTGCTCAC
GCATCTGCTGGATAAGCATTATGGTCTCACACTCAATGACACGCCGTTTGGCGACGACGGCGTAATTCAGGCGCACATCA
ACGCCGGTATCTCTCTGTGCGACGCCGTCAACTTCATTGTGGAAAGGTATGAACTGTTGCGCACTGACCACAGCAGCTCC
AGCCTCACCGAGCACTCTTCACTGATTGGTGCCATCGACATCCTGCGTGCCCACCGTGCAACCGGCCTGATAGTGGGCAG
CGGCTACAGCACCATCACCCGCATCACAACGGGCCAGTATCGGGAGGACAAAACACAGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT201529 WP_000450520.1 NZ_CP074121:88050-88277 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT201529 NZ_CP098486:3487339-3487665 [Enterobacter hormaechei subsp. hormaechei]
ATGCATTCTGAACAATGGGGACTGACGTGCGGTAAAACGCCGTGCTTCAGTGCCAAGCTGGTGCAGGAAGGCAACCGGCT
GCACTTTCTAGCTGACCGAGCAGAGTTTAGTGGCGACTTGAGCGACGATGAGGCACTGCGCCTCGACCAGGCTTTTCCGG
TGATATTGAAGCAGCTGGAACAAATGCTTTCAGCCGGTGAGCTGAATCCCCGCCATCAGCATCGCGTCACTCTGCATCTT
AACGGGCTAACCTGCGAAGCCGACACGCTCGGTTCGCACGGCTACCTTTACATCGCGATTTATCCCTCAGCGGTAGCTAC
CGGCTGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2