201518

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2548743..2549240 Replicon chromosome
Accession NZ_CP074120
Organism Escherichia coli strain EC16

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KGQ53_RS12275 Protein ID WP_001173343.1
Coordinates 2548743..2548916 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KGQ53_RS12280 Protein ID WP_000398619.1
Coordinates 2548944..2549240 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGQ53_RS12265 (2545413) 2545413..2546870 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
KGQ53_RS12270 (2547107) 2547107..2548624 + 1518 WP_001295074.1 lysine--tRNA ligase -
KGQ53_RS12275 (2548743) 2548743..2548916 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KGQ53_RS12280 (2548944) 2548944..2549240 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KGQ53_RS12285 (2549467) 2549467..2549739 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
KGQ53_RS12290 (2549751) 2549751..2549981 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
KGQ53_RS12295 (2550162) 2550162..2551793 + 1632 WP_001216477.1 sensor histidine kinase -
KGQ53_RS12300 (2551790) 2551790..2552509 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T201518 WP_001173343.1 NZ_CP074120:c2548916-2548743 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T201518 NZ_CP098478:447742-448131 [Acinetobacter sp. C26M]
ATGGCTAATGGTGTTTTCGAGCTGCAACGCAGTCGTTTTGCGATGGTATTGCAGCTCTTGATTTTCATTGCCATCCTTAG
TTTAACTTATTCCTTGCTTGCCTTATGGATTTGGCTATTGTGCTTCTGCATGATGGGAGCTGCTTGGATTTTGTTTCTCA
AACAGCCACAGATTAAACGTTTTGAATACTTGGATCATCAAGAATGTTCGTTCGAGTTTTATGACCCCGAATTAAAGATT
CAGCGCAGAAAAATCGTTAAAATATTGGATCACAAATTTTACATCGCACTCTATTTTTCTCATTCAAACCAGAGAACTTG
TATCATTTGGTGGGATCAATTATCACATTCTCAGTGGCAAAAGCTAAAATTACGCGCAAAATTGGCATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT201518 WP_000398619.1 NZ_CP074120:c2549240-2548944 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT201518 NZ_CP098478:447492-447749 [Acinetobacter sp. C26M]
ATGTCTGATGAAATGACACTGGAAGAGCGTAAGGTTGTGTATCGTGCACGTCGTGGTTTAAAAGAAATCGATGTGTATTT
TGATCCTTATGTTAAAAACCACTATCTGACAGCTGATATCGCTGAAAAGGCATTATTTGCGGAGTTGGTCGATCAAGAAG
ATCCTGATTTATTGGATTGGTTTATGGAGGTTGGAGAACCGCCTCGTGCAGAATTAAAAGATTTTATTTTAAAATTAAAA
CACTATGTCCATGGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References