201516

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2331970..2332546 Replicon chromosome
Accession NZ_CP074120
Organism Escherichia coli strain EC16

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag KGQ53_RS11180 Protein ID WP_001297643.1
Coordinates 2332259..2332546 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag KGQ53_RS11175 Protein ID WP_000063160.1
Coordinates 2331970..2332272 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGQ53_RS11160 (2328566) 2328566..2330716 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
KGQ53_RS11165 (2330766) 2330766..2330969 + 204 WP_000467859.1 YbdD/YjiX family protein -
KGQ53_RS11170 (2330980) 2330980..2331936 + 957 WP_001297640.1 GTPase -
KGQ53_RS11175 (2331970) 2331970..2332272 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
KGQ53_RS11180 (2332259) 2332259..2332546 - 288 WP_001297643.1 BrnT family toxin Toxin
KGQ53_RS26105 (2332745) 2332745..2333441 - 697 Protein_2214 IS1 family transposase -
KGQ53_RS11190 (2333756) 2333756..2334988 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
KGQ53_RS11195 (2335029) 2335029..2336309 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2332745..2332954 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T201516 WP_001297643.1 NZ_CP074120:c2332546-2332259 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T201516 NZ_CP098474:c1642811-1642629 [Neisseria gonorrhoeae]
TTGAATAGCCTAGACGTTATTGCCCTACTCAAACAAGACGGTTGGTATAAAGTTGCACAATCCGGGAGCCATTCGCAATA
TAAGCACCCAACAAAAAAAGGCCGTGTAACCGTGCCGCACCCGAAAAAAGACTTGCCGACAGGTACTGTAAAAAATATCT
ATAAGCAAGCCGGTTTGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT201516 WP_000063160.1 NZ_CP074120:c2332272-2331970 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT201516 NZ_CP098474:c1642527-1642126 [Neisseria gonorrhoeae]
ATGTTTATCCCTGCCGCCCTGCACAAAGACGAACATTCGGCATATGGCGTAACCATCCCCGACCTTCCCGGCTGTTTCTC
TTGCGGCGACACCGTTGAAGAAGCCGTCGCCAACGCCCGTTCTGCCGCCTATATGCATATTGACGGTATGATTGAGGACG
GAGGGTTCAAAAACCTTGCCGTAAGCAGCATTGCCGATTTGAGCCAAGAACCTGATTATCATGGGGCAACATGGGTAATG
ATTGAAATCGACCCTGCAAAAATCAGTCGGCAGCAAATCCGATTCAACGTCAGCTGGCCGCAATACCTTCTTGATAGAGT
GGATGAATATACATCCGCAAATCATGAAACCCGTAGCGGTTTTTTAGCCAAAGCCGCCCTGCTCACCATGAATCAGGCAT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References