Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1778862..1779480 Replicon chromosome
Accession NZ_CP074120
Organism Escherichia coli strain EC16

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KGQ53_RS08570 Protein ID WP_001291435.1
Coordinates 1779262..1779480 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KGQ53_RS08565 Protein ID WP_000344800.1
Coordinates 1778862..1779236 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGQ53_RS08555 (1773951) 1773951..1775144 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KGQ53_RS08560 (1775167) 1775167..1778316 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KGQ53_RS08565 (1778862) 1778862..1779236 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KGQ53_RS08570 (1779262) 1779262..1779480 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KGQ53_RS08575 (1779652) 1779652..1780203 + 552 WP_000102564.1 maltose O-acetyltransferase -
KGQ53_RS08580 (1780319) 1780319..1780789 + 471 WP_000136192.1 YlaC family protein -
KGQ53_RS08585 (1780953) 1780953..1782503 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KGQ53_RS08590 (1782545) 1782545..1782898 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KGQ53_RS08600 (1783277) 1783277..1783588 + 312 WP_000409911.1 MGMT family protein -
KGQ53_RS08605 (1783619) 1783619..1784191 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T201513 WP_001291435.1 NZ_CP074120:1779262-1779480 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T201513 NZ_CP098472:c1521668-1521486 [Neisseria gonorrhoeae]
TTGAATAGCCTAGACGTTATTGCCCTACTCAAACAAGACGGTTGGTATAAAGTTGCACAATCCGGGAGCCATTCGCAATA
TAAGCACCCAACAAAAAAAGGCCGTGTAACCGTGCCGCACCCGAAAAAAGACTTGCCGACAGGTACTGTAAAAAATATCT
ATAAGCAAGCCGGTTTGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT201513 WP_000344800.1 NZ_CP074120:1778862-1779236 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT201513 NZ_CP098472:c1521384-1520983 [Neisseria gonorrhoeae]
ATGTTTATCCCTGCCGCCCTGCACAAAGACGAACATTCGGCATATGGCGTAACCATCCCCGACCTTCCCGGCTGTTTCTC
TTGCGGCGACACCGTTGAAGAAGCCGTCGCCAACGCCCGTTCTGCCGCCTATATGCATATTGACGGTATGATTGAGGACG
GAGGGTTCAAAAACCTTGCCGTAAGCAGCATTGCCGATTTGAGCCAAGAACCTGATTATCATGGGGCAACATGGGTAATG
ATTGAAATCGACCCTGCAAAAATCAGTCGGCAGCAAATCCGATTCAACGTCAGCTGGCCGCAATACCTTCTTGATAGAGT
GGATGAATATACATCCGCAAATCATGAAACCCGTAGCGGTTTTTTAGCCAAAGCCGCCCTGCTCACCATGAATCAGGCAT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References