Detailed information of TA system TA201512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 748533..749171
• Replicon: chromosome
• Accession: NZ_CP074120
• Organism: Escherichia coli strain EC16

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: KGQ53_RS03615
• Protein ID: WP_001447010.1
• Coordinates: 748995..749171 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KGQ53_RS03610
• Protein ID: WP_001270286.1
• Coordinates: 748533..748949 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGQ53_RS03590 (743685)	743685..744626	-	942	WP_001251304.1	ABC transporter permease	-
KGQ53_RS03595 (744627)	744627..745640	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
KGQ53_RS03600 (745658)	745658..746803	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
KGQ53_RS03605 (747048)	747048..748454	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
KGQ53_RS03610 (748533)	748533..748949	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KGQ53_RS03615 (748995)	748995..749171	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KGQ53_RS03620 (749393)	749393..749623	+	231	WP_000494244.1	YncJ family protein	-
KGQ53_RS03625 (749715)	749715..751676	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KGQ53_RS03630 (751749)	751749..752285	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
KGQ53_RS03635 (752377)	752377..753552	+	1176	WP_249344232.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T201512 WP_001447010.1 NZ_CP074120:c749171-748995 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T201512 NZ_CP098472:651180-651599 [Neisseria gonorrhoeae]
ATGATTTTGCTGGACACGAATGTGATTTCCGAACCTTTGCGCCCACAACCCAATGAACGTGTGGTGGCATGGTTGGATAG
TTTGATATTGGAAGATGTGTATTTGTCTGCCATTACTGTTGCAGAATTGCGTTTGGGTGTGGCGTTGTTGCTCAATGGCA
AGAAAAAGAATGTGCTGCACGAACGTTTGGAACAATCCATTTTGCCTTTATTTGCGGGGCGGATTCTGCCTTTTGATGAA
CCGGTTGCCGCAATCTATGCGCAAATTCGTTCCTATGCCAAAACACATGGCAAAGAGATTGCTGCCGCAGACGGCTATAT
TGCCGCCACTGCAAAACAGCACAGTTTGACAGTTGCTACGCGTGATACCGGCTCATTTTTTGCGGCCGATGTCGCGGTGT
TCAATCCGTGGCACGATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT201512 WP_001270286.1 NZ_CP074120:c748949-748533 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT201512 NZ_CP098472:650944-651180 [Neisseria gonorrhoeae]
ATGGCTTCTGTTGTGATTAGAAATTTATCCGAGGCCACGCACAACGCAATCAAATTCCGTGCGCGAGCCGCAGGGCGCAG
TACCGAAGCAGAAATCCGCTTAATTTTGGATAACATCGCCAAAGCACAACAAACTGTACGTTTGGGGTCAATGTTGGCAT
CAATAGGGCAGGAAATCGGAGGTGTTGAGCTGGAAGACGTACGCGGTCGTAATACTGATAACGAGGTTTCTTTGTGA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8