Detailed information of TA system TA201500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 5150..5730
• Replicon: plasmid unnamed3
• Accession: NZ_CP074119
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain kpn-hnqyy

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: KGB32_RS28560
• Protein ID: WP_071177730.1
• Coordinates: 5150..5464 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: KGB32_RS28565
• Protein ID: WP_000093040.1
• Coordinates: 5452..5730 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGB32_RS28535 (KGB32_28535)	1094..3058	+	1965	WP_071177727.1	TraM recognition domain-containing protein	-
KGB32_RS28540 (KGB32_28540)	3058..3789	+	732	WP_071177728.1	MobC family replication-relaxation protein	-
KGB32_RS28545 (KGB32_28545)	3796..4326	+	531	WP_071177729.1	hypothetical protein	-
KGB32_RS28550 (KGB32_28550)	4353..4532	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
KGB32_RS28555 (KGB32_28555)	4558..4986	-	429	WP_001140599.1	hypothetical protein	-
KGB32_RS28560 (KGB32_28560)	5150..5464	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KGB32_RS28565 (KGB32_28565)	5452..5730	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
KGB32_RS28905	5905..6270	-	366	WP_072354022.1	TonB family protein	-
KGB32_RS28575 (KGB32_28575)	6267..6638	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
KGB32_RS28580 (KGB32_28580)	6912..7157	-	246	WP_032440458.1	hypothetical protein	-
KGB32_RS28585 (KGB32_28585)	7484..9169	+	1686	WP_032440457.1	colicin-like bacteriocin tRNase domain-containing protein	-
KGB32_RS28590 (KGB32_28590)	9179..9436	+	258	WP_032440455.1	colicin E3-like toxin immunity protein	-
KGB32_RS28595 (KGB32_28595)	9521..9670	+	150	WP_032440454.1	colicin release lysis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10060	10060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T201500 WP_071177730.1 NZ_CP074119:c5464-5150 [Klebsiella pneumoniae subsp. pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T201500 NZ_CP098464:c951043-950624 [Neisseria gonorrhoeae]
ATGATTTTGCTGGACACGAATGTGATTTCCGAACCTTTGCGCCCACAACCCAATGAACGTGTGGTGGCATGGTTGGATAG
TTTGATATTGGAAGATGTGTATTTGTCTGCCATTACTGTTGCAGAATTGCGTTTGGGTGTGGCGTTGTTGCTCAATGGCA
AGAAAAAGAATGTGCTGCACGAACGTTTGGAACAATCCATTTTGCCTTTATTTGCGGGGTGGATTCTGCCTTTTGATGAA
CCGGTTGCCGCAATCTATGCGCAAATTCGTTCCTATGCCAAAACACATGGCAAAGAGATTGCTGCCGCAGACGGCTATAT
TGCCGCCACTGCAAAACAGCACAGTTTGACAGTTGCTACGCGTGATACCGGCTCATTTTTTGCGGCCGATGTCGCGGTGT
TCAATCCGTGGCACGATTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT201500 WP_000093040.1 NZ_CP074119:c5730-5452 [Klebsiella pneumoniae subsp. pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT201500 NZ_CP098464:c951279-951043 [Neisseria gonorrhoeae]
ATGGCTTCTGTTGTGATTAGAAATTTATCCGAGGCCACGCACAACGCAATCAAATTCCGTGCGCGAGCCGCAGGGCGCAG
TACCGAAGCAGAAATCCGCTTAATTTTGGATAACATCGCCAAAGCACAACAAACTGTACGTTTGGGGTCAATGTTGGCAT
CAATAGGGCAGGAAATCGGAGGTGTTGAGCTGGAAGACGTACGCGGTCGTAATACTGATAACGAGGTTTCTTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1