Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5453238..5453863 Replicon chromosome
Accession NZ_CP074116
Organism Klebsiella pneumoniae subsp. pneumoniae strain kpn-hnqyy

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KGB32_RS26840 Protein ID WP_002882817.1
Coordinates 5453238..5453621 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KGB32_RS26845 Protein ID WP_004150355.1
Coordinates 5453621..5453863 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGB32_RS26825 (KGB32_26825) 5450604..5451506 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KGB32_RS26830 (KGB32_26830) 5451503..5452138 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KGB32_RS26835 (KGB32_26835) 5452135..5453064 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KGB32_RS26840 (KGB32_26840) 5453238..5453621 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KGB32_RS26845 (KGB32_26845) 5453621..5453863 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KGB32_RS26850 (KGB32_26850) 5454068..5454985 + 918 WP_002882812.1 alpha/beta hydrolase -
KGB32_RS26855 (KGB32_26855) 5454999..5455940 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KGB32_RS26860 (KGB32_26860) 5455985..5456422 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KGB32_RS26865 (KGB32_26865) 5456419..5457279 - 861 WP_002882807.1 virulence factor BrkB family protein -
KGB32_RS26870 (KGB32_26870) 5457273..5457872 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T201492 WP_002882817.1 NZ_CP074116:c5453621-5453238 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T201492 NZ_CP098458:657683-658102 [Neisseria gonorrhoeae]
ATGATTTTGCTGGACACGAATGTGATTTCCGAACCTTTGCGCCCACAACCCAATGAACGTGTGGTGGCATGGTTGGATAG
TTTGATATTGGAAGATGTGTATTTGTCTGCCATTACTGTTGCAGAATTGCGTTTGGGTGTGGCGTTGTTGCTCAATGGCA
AGAAAAAGAATGTGCTGCACGAACGTTTGGAACAATCCATTTTGCCTTTATTTGCGGGGCGGATTCTGCCTTTTGATGAA
CCGGTTGCCGCAATCTATGCGCAAATTCGTTCCTATGCCAAAACACATGGCAAAGAGATTGCTGCCGCAGACGGCTATAT
TGCCGCCACTGCAAAACAGCACAGTTTGACAGTTGCTACGCGTGATACCGGCTCATTTTTTGCGGCCGATGTCGCGGTGT
TCAATCCGTGGCACGATTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT201492 WP_004150355.1 NZ_CP074116:c5453863-5453621 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT201492 NZ_CP098458:657447-657683 [Neisseria gonorrhoeae]
ATGGCTTCTGTTGTGATTAGAAATTTATCCGAGGCCACGCACAACGCAATCAAATTCCGTGCGCGAGCCGCAGGGCGCAG
TACCGAAGCAGAAATCCGCTTAATTTTGGATAACATCGCCAAAGCACAACAAACTGTACGTTTGGGGTCAATGTTGGCAT
CAATAGGGCAGGAAATCGGAGGTGTTGAGCTGGAAGACGTACGCGGTCGTAATACTGATAACGAGGTTTCTTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References