Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4198950..4199569 Replicon chromosome
Accession NZ_CP074116
Organism Klebsiella pneumoniae subsp. pneumoniae strain kpn-hnqyy

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KGB32_RS20805 Protein ID WP_002892050.1
Coordinates 4199351..4199569 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KGB32_RS20800 Protein ID WP_002892066.1
Coordinates 4198950..4199324 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGB32_RS20790 (KGB32_20790) 4194102..4195295 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KGB32_RS20795 (KGB32_20795) 4195318..4198464 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
KGB32_RS20800 (KGB32_20800) 4198950..4199324 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KGB32_RS20805 (KGB32_20805) 4199351..4199569 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KGB32_RS20810 (KGB32_20810) 4199728..4200294 + 567 WP_002892030.1 maltose O-acetyltransferase -
KGB32_RS20815 (KGB32_20815) 4200266..4200406 - 141 WP_004147370.1 hypothetical protein -
KGB32_RS20820 (KGB32_20820) 4200427..4200897 + 471 WP_002892026.1 YlaC family protein -
KGB32_RS20825 (KGB32_20825) 4200872..4202323 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KGB32_RS20830 (KGB32_20830) 4202424..4203122 + 699 WP_002892021.1 GNAT family protein -
KGB32_RS20835 (KGB32_20835) 4203119..4203259 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KGB32_RS20840 (KGB32_20840) 4203259..4203522 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T201487 WP_002892050.1 NZ_CP074116:4199351-4199569 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T201487 NZ_CP098450:c3949110-3948733 [Proteus mirabilis]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCATCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT201487 WP_002892066.1 NZ_CP074116:4198950-4199324 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT201487 NZ_CP098450:c3949534-3949157 [Proteus mirabilis]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAGGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGCTTATCTGGCTGTTTATCCGACACCGGCAGAGCCCGCAATCACCGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References